Abstract
The emergence of heterogeneous cellular state in uniform environment was studied. Using a continuous culture system which provides homogeneous culture environment, we investigated the fluctuation in expression level of glnA gene in a cell population. As results, we found that the expression level of glnA gene in the cells exhibit a large fluctuation (with two orders of magnitude of protein number), even though expression of the gene is essential for cellular growth and the environment is homogeneous. Furthermore, among several steady states, the transient processes of such heterogeneous cell population were investigated, by changing environmental conditions. The results showed that cells can respond to environmental changes even when their intra-cellular state is accompanied by fluctuations. These results may provide a clue to understand why biological systems can maintain and reproduce themselves robustly.
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Yamada, T. et al. (2006). Analysis of Fluctuation in Gene Expression Based on Continuous Culture System. In: Ijspeert, A.J., Masuzawa, T., Kusumoto, S. (eds) Biologically Inspired Approaches to Advanced Information Technology. BioADIT 2006. Lecture Notes in Computer Science, vol 3853. Springer, Berlin, Heidelberg. https://doi.org/10.1007/11613022_12
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DOI: https://doi.org/10.1007/11613022_12
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-31253-6
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