Abstract
We describe a new high-throughput method to quantify the structural properties of individual cell-sized liposomes. We labeled an internal aqueous solution of liposomes with a green fluorescent protein (GFP) and the membrane with a fatty acid conjugated with a red fluorescent probe. The internal aqueous volume and lipid membrane volume of each liposome was measured, and double-labeled liposomes were analyzed by flow cytometry, a useful tool that enables us to estimate the internal aqueous and lipid membrane volumes of individual cell-sized liposomes independently of shape and structure. This method shows promise in opening the way to understanding the characteristics of biochemical reactions occurring within a liposome, to optimizing the preparation method of liposomes, and to overcoming many of the difficulties in realizing an artificial cell.
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Sato, K., Obinata, K., Sugawara, T., Urabe, I., Yomo, T. (2006). A High-Throughput Method to Quantify the Structural Properties of Individual Cell-Sized Liposomes by Flow Cytometry. In: Ijspeert, A.J., Masuzawa, T., Kusumoto, S. (eds) Biologically Inspired Approaches to Advanced Information Technology. BioADIT 2006. Lecture Notes in Computer Science, vol 3853. Springer, Berlin, Heidelberg. https://doi.org/10.1007/11613022_27
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DOI: https://doi.org/10.1007/11613022_27
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-31253-6
Online ISBN: 978-3-540-32438-6
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