Abstract
To investigate the accuracy for absolute quantification of brain creatine (Cr) concentration using in vivo long echo time PRESS sequence performed with an external standard. Ten swine and an external standard phantom were investigated by 1.5T GE Signa scanner and the standard head coil. 1H-MRS data were acquired from the two VOI (2x2x2cm3) placed in swine brain and external standard solution by using PRESS sequence with TE = 135 mses, TR = 1500 msec, and 128 scan averages. In vivo Cr evaluation was made by LCModel. In vitro Cr concentration was analyzed by HPLC method. In the 1H-MRS group, the Cr concentration was 9.37±0.137 mmol/kg. In the HPLC group, the Cr concentration was 8.905±0.126 mmol/kg. Good agreement was obtained between in these two methods (P=0.491), which indicated that long echo time PRESS sequence with an external standard can accurately detect brain Cr concentration. The application of LCModel introduces more convenience for the MRS quantification.
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References
Urenjak, J., Williams, S.R., Gadian, D.G.: Proton nuclear magnetic resonance spectroscopy unambiguously identifies different neural cell types. J. Neurosci. 13, 981–989 (1993)
Mich, T., Wick, M., Fuji, H.: Proton MRS of oral creatine supplementation in rats: cerebral metabolite concentrations and ischemic challenge. NMR Biomed. 12, 309–314 (1999)
Wilken, B., Ramirez, J.M., Probst, I.: Creatine protects the central respiratory network of mammals under anoxic conditions. Pediat. Re. 43, 8–14 (1998)
Stöckler, S., Hanefeld, F., Frahm, J.: Creatine replacement therapy in guanidinoacetate methyltransferase deficiency, a novel inborn error of metabolism. Lancet. 348, 789–790 (1996)
Wyss, M., Kaddurah-Daouk, R.: Creatine and creatinine metabolism. Physiol. Rev. 80, 1107–1213 (2000)
Lubec, B., Aufricht, C.: Creatine reduces collagen accumulation in the kidneys of diabetic db/db mice. Nephron. 67, 214–217 (1994)
Tong, Z., Yamaki, T., Herkner, K.: In vivo quantification of the metabolites in normal brain and brain tumors by proton MR spectroscopy using water as an internal standard. Magn. Reson. Imaging 22, 735–742 (2004)
Christiansen, P., Henriksen, O., Stubgaard, M.: In vivo quantification of brain metabolites by 1H-MRS using water as an internal standard. Magn. Reson. Imaging 11, 107–118 (1993)
Mich, T.: Absolute Concentrations of Metabolites in the Adult Human Brain in vivo: Quantification of Localized Proton MR Spectra. Radiology 187, 219–227 (1993)
Friedrich, G., Woermann, M.D., Mary, A.: Short Echo Time Single-Voxel 1H MagneticResonance Spectroscopy in Magnetic Resonance Imaging–Negative Temporal Lobe Epilepsy: Different Biochemical Profile Compared with Hippocampal Sclerosis. Ann. Neurol. 45, 369–376 (1999)
Woermann, F.G., Mclean, M.A.: Quantitative short echo time proton magnetic resonance spectroscopic imaging study of malformations of cortical development causing epilepsy. Brain 124, 427–436 (2001)
Provencher, S.W.: LCModel User’s Manual, version: 6.1-6.4 (2005)
DeCarli, C., Maisog, J., Murphy, D.G.: Method for quantification of brain, ventricular, and subarachnoid CSF volumes from MR images. Journal of Computer Assisted Tomography 16, 274–284 (1992)
Sun, A.M., Wang, E.R., Mao, B.Y.: Determination of creatine Phosphocreatine and Adenosinephosphates in Experimental Hydrocephalus Tissue by Reversed-Phase High Performance Liquid Chromatography. J. Sichuan. Univ. (Med. Sci. Edi.) 35, 113–116 (2004)
Li, B.S., Wang, H.: Metabolite ratios to assumed stable creatine level confound the quantification of proton brain MR spectroscopy. Magn. Reson. Imaging 21, 923–928 (2003)
Keevil, S.F., Barbiroli, B., Brooks, J.W.C.: Absolute metabolite quantification by in vivo NMR Spetroscopy: II. A multicentre trial of protocolis for in vivo localized proton studies of human brain. Magnetic Resonance Imaging 16, 1093–1106 (1998)
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Lin, Y. et al. (2006). Absolute Quantification of Brain Creatine Concentration Using Long Echo Time PRESS Sequence with an External Standard and LCModel: Verification with in Vitro HPLC Method. In: King, I., Wang, J., Chan, LW., Wang, D. (eds) Neural Information Processing. ICONIP 2006. Lecture Notes in Computer Science, vol 4232. Springer, Berlin, Heidelberg. https://doi.org/10.1007/11893028_23
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DOI: https://doi.org/10.1007/11893028_23
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