Abstract
The paper demonstrates experimentally the basic principle of DNA transfer between magnetic bead based selection stages, which can be used in steady flow microreactors for DNA Computing [McCaskill, J.S.: Biosystems, 59 (2001) 125–138] and molecular diagnostics. Short DNA oligomers, which can be attached covalently to magnetic beads by a light programmable photochemical procedure [Penchovsky et.al.: Nucleic Acids Res., 22 (2000) e98], are used to bind matching ssDNA from a flowing solution. The beads are restrained in two reaction chambers (modules) by etched ledges in a bonded microreactor made of silicon and glass, with the solutions flowing in closed micro-channels. The action of a steady flow network of selection modules is studied in this two chamber microreactor using a succession of different buffer solutions at alternate pH to simulate the transfer between parallel flows in the former system. The pH changes cause successive hybridisation and dissociation of ssDNA to matching sequences on the beads. Detection of DNA is by fluorescence from rhodamine-labelled target DNA. The results demonstrate the successful selection of specific DNA in one module and its subsequent transfer to and pickup on the magnetic beads of a second module. This verifies the biochemical operation of the basic processing step for optically programmable DNA Computing in micro-flow reactors.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
McCaskill, J.S.: Biosystems, 59 (2001) 125–138.
McCaskill, J., Penchovsky, R., Gohlke, M., Ackermann, J. and Ruecker, T.: DNA6 Sixth International Meeting on DNA Based Computers-Preliminary Proceedings. Leiden Center for Natural Computing, The Netherlands (2000) 239–246.
Penchovsky, R., Birch-Hirschfeld, E. and McCaskill, J.S.: Nucleic Acids Res., 22 (2000) e98.
Van Noort, D., Gast, F.U. and McCaskill, J.S.: DNA Computing in Microreactors, DNA7 Conference Proceedings (2001).
Frutos, A. G, et.al.: Nucleic Acids Res., 25 (1997) 4748–4757.
Adleman, L.M.: Science, 266 (1994) 1021–1024.
Hadd, A.G., Raymound, D.E., Hailiwell, J.W., Jacobson, S.C., Ramsey, J.M.: Anal. Chem., 69 (1997) 3407–3412.
Hossein, S., Tang, T., Harrison, D.J.: J. Am. Chem. Soc., 119, 8716–8717 (1997).
Kopp, M.U., de Mello, A. J. and Manz, A.: Science, 280, 1046–1048 (1998).
Burns, M.A., Johnson, B.N., Brahmasandra, S.N., Handique, K., Webster, J.R., Krishnan, M., Sammarco, T.S., Man, P.M., Jones, D., Heldsinger, D., Mastragelo, C.H., Burke, D.T.: Science, 282 (1998) 484–487.
Han, J. and Craighead, H. G.: Science, 288 (2000) 1026–1029.
Hugh Fan, Z., Mangru, S., Granzow, R., Heaney, P., Ho, W., Dong, Q. and Kumar, R.: Anal. Chem., 71 (1999) 4851–4859.
Chui, D.T., Pezzoli, E., Wu, H., Stroock, A.D. and Whitesides, G.M.: PNAS, 98 (2001) 2961–2966.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Penchovsky, R., McCaskill, J.S. (2002). Cascadable Hybridisation Transfer of Specific DNA between Microreactor Selection Modules. In: Jonoska, N., Seeman, N.C. (eds) DNA Computing. DNA 2001. Lecture Notes in Computer Science, vol 2340. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-48017-X_5
Download citation
DOI: https://doi.org/10.1007/3-540-48017-X_5
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-43775-8
Online ISBN: 978-3-540-48017-4
eBook Packages: Springer Book Archive