Abstract
New recognition probes sensible, specific and robust is one of the major problems of biosensor assay. Detection biosensors has utilized antibodies or enzymes as bioreceptors; however, these have numerous disadvantages of limited binding sites and physico-chemical instabilities, can negatively affect capture and detection of target in diagnostic device. In this contest, Phage-Display provides a valuable technique for obtaining large amounts of specific and robustness bio-probes in a relatively short time. This technique relies on the ability of M13 bacteriophages (or phages) to display specific and selective target-binding peptides on major coat protein pVIII of their surface. In this work, we used P9b phage clone, displaying a foreign peptide QRKLAAKLT to selectively recognize Pseudomonas aeruginosa like bioreceptor. We describe different methods of functionalization to realize a selective bacteria biosensor surfaces. Several surfaces, such as latex and magnetic beads and polymeric surfaces such as mica, APTES and PEI, were functionalized by covalent bonds or physisorption with P9b. The efficiency of the surface functionalization procedures was evaluated by ELISA and AFM, while capture efficiency of the anchored phages has been assessed by plate count and Fluorescence microscopy. The results of this work pave the way to the use of phages as bioreceptor.
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Sposito, A.J., Kurdekar, A., Zhao, J., Hewlett, I.: Application of nanotechnology in biosensors for enhancing pathogen detection. WIREs Nanomed. Nanobiotechnol. (2018). https://doi.org/10.1002/wnan.1512
Alhadrami, H.A.: Biosensors: Classifications, medical applications, and future prospective. Biotechnol. Appl. Biochem. 2017
Chen, J., Duncan, B., Wang, Z., Wang, L.-S., Rotello, V.M., Nugen, S.R.: Bacteriophage-based nanoprobes for rapid bacteria separation. https://doi.org/10.1039/c5nr03779d
Qi, H., Lu, H., Qiu, H.J., Petrenko, V.A., Liu, A.: Phagemid vectors for phage display: properties, characteristics and construction. J. Mol. Biol. 417(3), 129–143 (2012). https://doi.org/10.1016/j.jmb.2012.01.038
Carnazza, S., Gioffrè, G., Felici, F., Guglielmino, S.: Recombinant phage probes for Listeria monocytogenes. J. Phys. Condens. Matter 19, 395011 (13 pp) (2007). http://dx.doi.org/10.1088/0953-8984/19/39/395011
Liu, P., Han, L., Wanga, F., Petrenko, V.A., Liu, A.: Gold nanoprobe functionalized with specific fusion protein selection from phage display and its application in rapid, selective and sensitive colorimetric biosensing of Staphylococcus aureus. Biosens. Bioelectron. 82, 195–203 (2016). https://doi.org/10.1016/j.bios.2016.03.075
Butler, J.C., Angelini, T., Tang, J.X., Wong, G.C.L.: Ion multivalence and like-charge polyelectrolyte attraction. Phys. Rev. Lett. 91, 028301 (2003)
Petrenko, V.A., Vodyanoy, V.J.: Phage display for detection of biological threat agents. J. Microbiol. Methods 53, 253–262 (2003) (PMID: 12654496)
Huang, S., Yang, H., Lakshmanan, R.S., Johnson, M.L., Chen, I., Wan, J., Wikle, H.C., Petrenko, V.A, Barbaree, J.M., Cheng, Z.Y.: The effect of salt and phage concentrations on the binding sensitivity of magnetoelastic biosensors for Bacillus anthracis detection. In: Chin, B.A. (ed.) Biotechnol. Bioeng. 101, 1014–1021 (2008)
Carnazza, S., Foti, C., Gioffrè, G., Felici, F., Guglielmino, S.: Specific and selective probes for Pseudomonas aeruginosa from phage-displayed random peptide libraries. Biosens. Bioelectron. 23, 1137–1144 (2008)
De Plano, L.M., Carnazza, S., Messina, G.M.L., Rizzo, M.G., Marletta, G., Guglielmino, S.P.P.: Specific and selective probes for Staphylococcus aureus from phage-displayed random peptide libraries. Colloids Surf. B Biointerfaces 157, 473–480 (2017)
Calabrese, F., Carnazza, S., De Plano, L.M., Lentini, G., Franco, D., Guglielmino, S.P.P.: Phage-coated paramagnetic beads as selective and specific capture system for biosensor applications. In: XVIII AISEM Annual Conference (2015)
Lentini, G., Franco, D., Fazio, E., De Plano, L.M., Trusso, S., Carnazza, S., Neri, F., Guglielmino, S.P.P.: Rapid detection of Pseudomonas aeruginosa by phage-capture system coupled with micro-Raman spectroscopy. Vib. Spectrosc. 86, 1–7 (2016). http://dx.doi.org/10.1016/j.vibspec.2016.05.003
Zimmermann, K., Hagedorn, H., Heucks, C.Chr., Hinrichsen, M., Ludwig, H.: The ionic properties of the filamentous bacteriophages Pfl and fd. J. Biol. Chem. 261(4), 1653–1655 (1986)
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De Plano, L.M. et al. (2019). M13 Bacteriophages as Bioreceptors in Biosensor Device. In: Andò, B., et al. Sensors. CNS 2018. Lecture Notes in Electrical Engineering, vol 539. Springer, Cham. https://doi.org/10.1007/978-3-030-04324-7_20
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DOI: https://doi.org/10.1007/978-3-030-04324-7_20
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