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International Conference on Applications in Electronics Pervading Industry, Environment and Society

ApplePies 2016: Applications in Electronics Pervading Industry, Environment and Society pp 138–144Cite as

Failure Effect Analysis of Patch-Clamp Electronic Instrumentation in Electrophysiology Experiments

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Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 429))

Abstract

The patch-clamp technique is an extremely powerful and exquisitely sensitive means for studying the biophysics and pharmacology of ion channels. Aiming at acquiring ionic currents, in the range from few pA to nA, while controlling the voltage across the membrane of a biological cell and continuously perfusing physiological solutions containing the molecules under investigation, patch-clamp measurements require: an accurate calibration phase, a low noise front-end, and a temperature control. After describing a patch-clamp electronic system, the paper discusses the most frequently encountered and well-fitting examples of how failures in the electronic instrumentation are related to errors and/or accuracy degradation in the electrophysiological measurement. The paper refers to experimental data recorded with this technique under the whole-cell voltage-clamp configuration.

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Author information

Authors and Affiliations

  1. Dip. Ingegneria della Informazione, Università di Pisa, via G. Caruso 16, Pisa, Italy

    Sergio Saponara & Massimo Macucci

  2. Dipartimento Scienze della Vita, Università di Siena, Via A. Moro 2, Siena, Italy

    Fabio Fusi & Simona Saponara

Authors
  1. Sergio Saponara
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  2. Fabio Fusi
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  3. Simona Saponara
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  4. Massimo Macucci
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Corresponding author

Correspondence to Sergio Saponara .

Editor information

Editors and Affiliations

  1. University of Genova , Genova, Italy

    Alessandro De Gloria

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© 2018 Springer International Publishing AG

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Cite this paper

Saponara, S., Fusi, F., Saponara, S., Macucci, M. (2018). Failure Effect Analysis of Patch-Clamp Electronic Instrumentation in Electrophysiology Experiments. In: De Gloria, A. (eds) Applications in Electronics Pervading Industry, Environment and Society. ApplePies 2016. Lecture Notes in Electrical Engineering, vol 429. Springer, Cham. https://doi.org/10.1007/978-3-319-55071-8_18

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  • DOI: https://doi.org/10.1007/978-3-319-55071-8_18

  • Published:

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-55070-1

  • Online ISBN: 978-3-319-55071-8

  • eBook Packages: EngineeringEngineering (R0)

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