Abstract
The mechanical properties of myocardial tissue are primarily determined by the organisation of the collagen network. Quantitative measurements of collagen morphology can help to understand the structure-function relationship in cardiac tissue. In this study, we segmented collagen from high-resolution three-dimensional (3D) images of the left ventricle (LV) mid-wall myocardium obtained using extended-volume confocal microscopy. 3D shape analysis was used to compute the morphological parameters elongation (e), flatness (f), and anisotropy (a). We applied this analysis to both control and hypertensive rat hearts and showed distinct differences between the control and remodelled hearts, particularly in collagen elongation. The predominant form of collagen in the control rat is elongated with a value of e = 0.846 ± 0.041, whereas in the hypertensive rat collagen, is arranged mostly in a sheet-like form with e = 0.301 ± 0.023. Such quantitative information can be used to develop microstructural models of the myocardium that link the observed changes in cardiac microstructure to changes in mechanical function during the progression of heart diseases, which will help to elucidate the underlying pathological mechanisms.
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References
Weber, K.T., Sun, Y., Tyagi, S.C., Cleutjens, J.P.: Collagen network of the myocardium: function, structural remodeling and regulatory mechanisms. J. Mol. Cell. Cardiol. 26, 279–292 (1994)
Berk, B.C., Fujiwara, K., Lehoux, S.: ECM remodeling in hypertensive heart disease. J. Clin. Invest. 117, 568–575 (2007)
Omens, J.H., Miller, T.R., Covell, J.W.: Relationship between passive tissue strain and collagen uncoiling during healing of infarcted myocardium. Cardiovasc. Res. 33, 351–358 (1997)
Kato, S., Spinale, F.G., Tanaka, R., Johnson, W., Cooper, G., Zile, M.R.: Inhibition of collagen cross-linking: effects on fibrillar collagen and ventricular diastolic function. Am. J. Physiol. Heart Circulatory Physiol. 269, H863–H868 (1995)
Brilla, C.G., Janicki, J., Weber, K.: Cardioreparative effects of lisinopril in rats with genetic hypertension and left ventricular hypertrophy. Circulation 83, 1771–1779 (1991)
LeGrice, I.J., Pope, A.J., Sands, G.B., Whalley, G., Doughty, R.N., Smaill, B.H.: Progression of myocardial remodeling and mechanical dysfunction in the spontaneously hypertensive rat. Am. J. Physiol. Heart Circulatory Physiol. 303, H1353–H1365 (2012)
Sands, G.B., Gerneke, D.A., Hooks, D.A., Green, C.R., Smaill, B.H., LeGrice, I.J.: Automated imaging of extended tissue volumes using confocal microscopy. Microsc. Res. Tech. 67, 227–239 (2005)
Young, A., LeGrice, I., Young, M., Smaill, B.: Extended confocal microscopy of myocardial laminae and collagen network. J. Microsc. 192, 139–150 (1998)
Weickert, J., Romeny, B.T.H., Viergever, M.A.: Efficient and reliable schemes for nonlinear diffusion filtering. IEEE Trans. Image Process. 7, 398–410 (1998)
Kapur, J.N., Sahoo, P.K., Wong, A.K.: A new method for gray-level picture thresholding using the entropy of the histogram. Comput. Vision Graph. Image Process. 29, 273–285 (1985)
Rangayyan, R.M.: Biomedical Image Analysis. CRC Press, Boca Raton (2004)
Loncaric, S.: A survey of shape analysis techniques. Pattern Recogn. 31, 983–1001 (1998)
Flusser, J., Zitova, B., Suk, T.: Moments and Moment Invariants in Pattern Recognition. Wiley, Hoboken (2009)
El Moumen, A., Kanit, T., Imad, A., El Minor, H.: Effect of reinforcement shape on physical properties and representative volume element of particles-reinforced composites: Statistical and numerical approaches. Mech. Mater. 83, 1–16 (2015)
Pope, A.J., Sands, G.B., Smaill, B.H., LeGrice, I.J.: Three-dimensional transmural organization of perimysial collagen in the heart. Am. J. Physiol. Heart Circulatory Physiol. 295, H1243–H1252 (2008)
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Hasaballa, A.I. et al. (2017). Three-Dimensional Quantification of Myocardial Collagen Morphology from Confocal Images. In: Pop, M., Wright, G. (eds) Functional Imaging and Modelling of the Heart. FIMH 2017. Lecture Notes in Computer Science(), vol 10263. Springer, Cham. https://doi.org/10.1007/978-3-319-59448-4_1
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DOI: https://doi.org/10.1007/978-3-319-59448-4_1
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