Abstract
Recently, micro robots driven by muscle cells attract a lot of attention. The shape of the robot can be controlled by modifying the growing of cells. This paper proposes a new cell patterning method by vibratory stimuli. The stimuli can detach redundant cells, and a certain cell pattern can be formed. In the experiment, around 80 Hz vibratory stimulus was applied to skeletal muscle cells via a PDMS scaffold and the cells were observed by camera. As a result, we found that a vibratory stimulus promotes detaching of the cells, especially at the moment of cell division. We also found that the increment of the cells depends on the vibratory energy. These results suggested that we can control the number of cells by vibration stimulus. Actually, we made specific cell pattern by the vibration stimulus.
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References
Shimizu, M., Yawata, S., Miyamoto, K., Miyasaka, K., Asano, T., Yoshinobu, T., Yawo, H., Ogura, T., Ishiguro, A.: Toward biorobotic systems with muscle cell actuators. In: The Proceedings of AMAM, pp. 87–88 (2011)
Kim, J., Park, J., Yang, S., Baek, J., Kim, B., Lee, S.H., Yoon, E.-S., Chun, K., Park, S.: Establishment of a fabrication method for a long-term actuated hybrid cell robot. Lab Chip 7(11), 1504–1508 (2007)
Park, S.-J., Gazzola, M., Park, K.S., Park, S., Di Santo, V., Blevins, E.L., Lind, J.U., Campbell, P.H., Dauth, S., Capulli, A.K., et al.: Phototactic guidance of a tissue-engineered soft-robotic ray. Science 353(6295), 158–162 (2016)
Singhvi, R., Kumar, A., Lopez, G.P., Stephanopoulos, G.N., Wang, D.I.C., Whitesides, G.M., Ingber, D.E.: Engineering cell shape and function. Science 696 (1994). New York then Washington
Yokoyama, S., Matsui, T.S., Deguchi, S.: Microcontact peeling as a new method for cell micropatterning. PloS One 9(7), e102735 (2014)
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Tashiro, I., Shimizu, M., Hosoda, K. (2017). Cell Patterning Method by Vibratory Stimuli. In: Mangan, M., Cutkosky, M., Mura, A., Verschure, P., Prescott, T., Lepora, N. (eds) Biomimetic and Biohybrid Systems. Living Machines 2017. Lecture Notes in Computer Science(), vol 10384. Springer, Cham. https://doi.org/10.1007/978-3-319-63537-8_59
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DOI: https://doi.org/10.1007/978-3-319-63537-8_59
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