Abstract
The fluorescence microscopy is suitable to observe a plane surface of an object but, when it comes to observe a three-dimensional structure of an object, light emissions from structures at different depth are mixed and it is difficult to isolate a section image on a target plane. Various techniques have been proposed to solve this problem but current techniques require an unaffordable computation cost and simplified techniques to save the cost, such as the nearest neighbor method, only produce low quality images. In this paper, we propose a technique that separates the out-of-focus-effect from front planes and that from behind planes. We evaluated effectiveness of this technique through some experiments and showed improved results under reduced computational cost.
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© 2004 Springer-Verlag Berlin Heidelberg
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Yano, K., Kumazawa, I. (2004). A Modified Nearest Neighbor Method for Image Reconstruction in Fluorescence Microscopy. In: Campilho, A., Kamel, M. (eds) Image Analysis and Recognition. ICIAR 2004. Lecture Notes in Computer Science, vol 3212. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-30126-4_2
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DOI: https://doi.org/10.1007/978-3-540-30126-4_2
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-23240-7
Online ISBN: 978-3-540-30126-4
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