Abstract
Embryo cultures of rodents is an established technique for monitoring adverse effects of chemicals on embryonic development. The assessment involves determination of the heart rate of the embryo which is usually done visually, a technique which is tedious and error prone. We present a new method for fully automatic heart detection in digital videos of rat embryos. First it detects the heart location, and then it counts the number of heart beats for a predetermined period of time. Using this automated method many more embryos can be evaluated at reasonable cost.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Flick, B., Klug, S.: Whole Embryo Culture: An Important Tool in Developmental Toxicology Today. Current Pharmaceutical Design 12, 1467–1488 (2006)
Robkin, M.A., Shepard, T.H., Tanimura, T.: A New in Vitro Culture Technique for Rat Embryos. Teratology 5, 367–376 (1972)
Sköld, A.C., Danielsson, C., Linder, B., Danielsson, B.R.: Teratogenicity of the IKr-Blocker Cisapride: Relation to Embryonic Cardiac Arrhythmia. Reproductive Toxicology 16, 333–342 (2002)
Danielsson, C., Azarbayjani, F., Sköld, A.C., Sjögren, K., Danielsson, B.R.: Polytherapy with hERG-blocking Antiepileptic Drugs: Increased Risk for Embryonic Cardiac Arrhythmia and Teratogenicity. Birth Defects Research Part A: Clinical and Molecular Teratology 79, 595–603 (2007)
Umbaugh, S.E.: Computer Vision and Image Processing: A Practical Approach Using CVIP Tool. Prentice Hall International, New Jersey (1998)
Dunn, D., Higgins, W.E.: Optimal Gabor Filters for Texture Segmentation. IEEE Transactions on Image Processing 4, 947–964 (1995)
Khan, M.A.U., Khan, M.K., Khan, M.A.: Coronary Angiogram Image Enhancement using Decimation-free Directional Filter Banks. In: IEEE International Conference on Acoustics, Speech, and Signal Processing, pp. 441–444. IEEE Press, Montreal (2004)
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 2008 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Khan, M.K., Nilsson, M.F., Danielsson, B.R., Bengtsson, E. (2008). Fully Automatic Heart Beat Rate Determination in Digital Video Recordings of Rat Embryos. In: Perner, P., Salvetti, O. (eds) Advances in Mass Data Analysis of Images and Signals in Medicine, Biotechnology, Chemistry and Food Industry. MDA 2008. Lecture Notes in Computer Science(), vol 5108. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-70715-8_3
Download citation
DOI: https://doi.org/10.1007/978-3-540-70715-8_3
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-70714-1
Online ISBN: 978-3-540-70715-8
eBook Packages: Computer ScienceComputer Science (R0)