Abstract
Automated analysis of molecular images has increasingly become an important research in computational life science. In this paper some new and efficient algorithms for detecting and analyzing cell phases of high-content screening are presented. The conceptual frameworks are based on the morphological features of cell nuclei. Furthermore, the novel detecting and analyzing strategies of feed-forward and feed-back of cell phases are proposed based on grey feature, cell shape, geometrical features and difference information of corresponding neighbor frames. Experiment results tested the efficiency of the new method.
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References
Fox, S.: Accommodating cells in HTS. Drug Discovery World 5, 21–30 (2003)
Feng, Y.: Practicing cell morphology based screen. European Pharmaceutical Review 7, 7–11 (2002)
Yarrow, J.C., et al.: Phenotypic screening of small molecule libraries by high throughput cell imaging. Comb. Chem. High Throughput Screen 6, 279–286 (2003)
Pham, T.D., Tran, D., Zhou, X., Wong, S.T.C.: An automated procedure for cellphase imaging identification. In: Proc. AI 2005 Workshop on Learning Algorithms for Pattern Recognition, pp. 52–29 (2005)
Pham, T.D., Tran, D.T., Zhou, X., Wong, S.T.C.: Classification of cell phases in time-lapse images by vector quantization and Markov models. In: Greer, E.V. (ed.) Neural Stem Cel l Research, Nova Science, New York (2006)
Davies, E.: Machine Vision: Theory, Algorithms and Practicalities, pp. 26–27, 79–99. Academic Press, London (1990)
Ostu, N.A.: Thresholding selection method from graylevel histogram. IEEE Trans. Systems Man Cybernet SMC8, 62–66 (1978)
Yu, D., Yan, H.: An efficient algorithm for smoothing, linearization and detection of structure feature points of binary image contours. Patt. Recog. 30(1), 57–69 (1997)
Yu, D., Pham, T.D., Zhou, X.: Analysis and Recognition of Touching Cell Images Based on Morphological Structures, Computational and Information Science. In: Apolloni, B., Howlett, R.J., Jain, L. (eds.) KES 2007, Part I. LNCS (LNAI), vol. 4692, pp. 439–446. Springer, Heidelberg (2007)
Fitzgibbon, A., Pilu, M., Fisher, R.B.: Direct Least Square Fitting of Ellipse. IEEE Trans. Pattern Analysis and Machine Intelligence 21, 476–480 (1999)
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Yu, D., Pham, T.D., Zhou, X. (2008). Detection and Analysis of Cell Nuclear Phases. In: Lovrek, I., Howlett, R.J., Jain, L.C. (eds) Knowledge-Based Intelligent Information and Engineering Systems. KES 2008. Lecture Notes in Computer Science(), vol 5177. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-85563-7_52
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DOI: https://doi.org/10.1007/978-3-540-85563-7_52
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-85562-0
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