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Identification of Proteins from Tuberculin Purified Protein Derivative (PPD) with Potential for TB Diagnosis Using Bioinformatics Analysis

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Advances in Bioinformatics and Computational Biology (BSB 2009)

Part of the book series: Lecture Notes in Computer Science ((LNBI,volume 5676))

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Abstract

The PPD is widely used for diagnosis of bovine tuberculosis, however it lacks specificity and sensitivity, generally attributed the cross-reactions with antigens shared by other Mycobacterium species. These highlight the necessity of better diagnostic tools to detect tuberculosis in cattle. We have identified proteins present in PPD preparations (avium and bovine PPD) by LC-MS/MS (Liquid Chromatography/Mass Spectrometry/Mass Spectrometry). A total of 169 proteins were identified. From these, four PPD proteins identified in bovine PPD and absent in avium PPD (Mb1961c, Mb2898, Mb2900 and Mb2965c) were select for bioinformatics analysis to identify an antigen with potential for TB diagnosis. We identified Mb1961c, a secreted protein, which has low identity proteins found in environmental mycobacteria. This protein could be used for the development of a diagnostic test or a synthetic PPD for bovine tuberculosis.

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Authors and Affiliations

  1. Centro de Biotecnologia, Universidade Federal de Pelotas, CP 354, 96010-900, Pelotas, RS, Brazil

    Sibele Borsuk, Fabiana Kommling Seixas, Daniela Fernandes Ramos, Caroline Rizzi & Odir Antonio Dellagostin

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  1. Sibele Borsuk
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  2. Fabiana Kommling Seixas
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  3. Daniela Fernandes Ramos
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  4. Caroline Rizzi
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  5. Odir Antonio Dellagostin
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Editor information

Editors and Affiliations

  1. Center of Informatics, Av. Prof. Luiz Freire, Federal University of Pernambuco, s/n, Cidade Universitária, PE 50740-540, Recife, Brazil

    Katia S. Guimarães

  2. National Library of Medicine, National Institutes of Health, National Center for Biotechnology Information, 8600 Rockville Pike, Building 38A 8S814, Bethesda, MD 20894, USA

    Anna Panchenko

  3. National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, 8600 Rockville Pike, Building 38A 8S814, MD 20894,, Bethesda, USA

    Teresa M. Przytycka

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© 2009 Springer-Verlag Berlin Heidelberg

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Borsuk, S., Seixas, F.K., Ramos, D.F., Rizzi, C., Dellagostin, O.A. (2009). Identification of Proteins from Tuberculin Purified Protein Derivative (PPD) with Potential for TB Diagnosis Using Bioinformatics Analysis. In: Guimarães, K.S., Panchenko, A., Przytycka, T.M. (eds) Advances in Bioinformatics and Computational Biology. BSB 2009. Lecture Notes in Computer Science(), vol 5676. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-03223-3_15

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  • DOI: https://doi.org/10.1007/978-3-642-03223-3_15

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-03222-6

  • Online ISBN: 978-3-642-03223-3

  • eBook Packages: Computer ScienceComputer Science (R0)

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