Abstract
Human Immunodeficiency Type-1 subtype C (HIV-1C) is rapidly diverging among populations causing more than 48% of infections worldwide. HIV-1C gp120’s 128 sequences available at Genbank were aligned and submitted to phylogenetic analysis. Three major clusters were identified: 72 sequences aligned with a Brazilian 0072eference sequence; 44 sequences aligned with an Ethiopian sequence and 12 could be group along with Indian isolates. A search was made for conserved HIV-1C cytotoxic T lymphocyte (CTL) epitopes to A*0201, A*0301, A*1101 e B*07 human leukocyte antigen (HLA) alleles (using Epijen software). Five most conserved epitopes were recognized: QMHEDIISL, CTHGIKPVV, NLTNNVKTI, AITQACPKV, CTRPNNNTR. Our results showed a recognized evolutionary force of HIV-1 to escape from CTL responses mutating sites that can be negatively select by host’s immune system. The present study brings up a new approach to in silico epitope analysis taking into account geographical informations on virus diversity and host genetic background.
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References
McMichael, A.J., Rowland-Jones, S.L.: Cellular Immune Responses to HIV. Nature 410, 980–987 (2001)
Novitsky, V.A., Montano, M.A., McLane, M.F., et al.: Molecular cloning and phylogenetic analysis of human immunodeficiency virus type I subtype C: a set of 23 full-length clones from Botswana. J. Virol. 73, 4427–4432 (1999)
Russell, K.L., Carcamo, C., Watts, D.M., et al.: Emerging genetic diversity of HIV-1 in South America. AIDS 14, 1785–1791 (2000)
Bello, G., Passaes, C.P., Guimaraes, M.L., Lorete, R.S., et al.: Origin and evolutionary history of HIV-1 subtype C in Brazil. AIDS 22, 1993–2000 (2008)
WHO-UNAIDS Report from a meeting Vaccine Advisory Comitee Geneva. Approaches to the development of broadly protective HIV vaccines: challenges posed by the genetic, biological and antigenic variability of HIV-1. AIDS 15, W1–W25 (2001)
De Groot, A.S., Jesdale, B., Martin, W., et al.: Mapping cross-clade HIV-1 vaccine epitopes using a bioinformatics approach. Vaccine 21, 4486–4504 (2003)
Oliveira, T., Deforche, K., Cassol, S., et al.: An Automated Genotyping System for Analysis of HIV-1 and other Microbial Sequences. Bioinfomatics 21(19), 3797–3800 (2005)
Rhee, S.Y., Gonzales, M.J., Kantor, R., et al.: Human immunodeficiency virus reverse transcriptase and protease sequence database. Nucleic Acids Res. 31, 298–303 (2003)
Thompson, J.D., Gibson, T.J., Plewniak, F., et al.: The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 24, 4876–4882 (1997)
Hall, T.A.: BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. In: Nucl. Acids Symp. Ser., vol. 41, pp. 95–98 (1999)
Swofford, D.L.: PAUP*: phylogenetic analysis using parsimony (*and other methods) Sinauer Associates, Sunderland, Mass. (2001)
Doytchinova, I.A., Guan, P., Flower, D.R.: EpiJen: a server for multi-step T cell epitope prediction. BMC Bioinformatics 7, 131 (2006)
Sette, A., Sidney, J.: Nine major HLA class I supertypes account for the vast preponderance of HLA-A and -B polymorphism. Immunogenetics 50, 201–212 (1999)
Kesmir, C., Nussbaum, A., Schild, H., Detours, V., Brunak, S.: Prediction of proteasome cleavage motifs by neural networks. Prot. Eng. 15(4), 287–296 (2002)
Shankar, P., Fabry, J.A., Fong, D.M., et al.: Three regions of HIV-1 gp160 contain clusters of immunodominant CTL epitopes. Immunol. Lett. 52, 23–30 (1996)
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Junqueira, D.M., de Medeiros, R.M., de Matos Almeida, S.E., Paixão-Cortez, V.R., Roehe, P.M., Spilki, F.R. (2009). Mapping HIV-1 Subtype C gp120Epitopes Using a Bioinformatic Approach. In: Guimarães, K.S., Panchenko, A., Przytycka, T.M. (eds) Advances in Bioinformatics and Computational Biology. BSB 2009. Lecture Notes in Computer Science(), vol 5676. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-03223-3_16
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DOI: https://doi.org/10.1007/978-3-642-03223-3_16
Publisher Name: Springer, Berlin, Heidelberg
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