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Micro Nucleus Detection in Human Lymphocytes Using Convolutional Neural Network

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Artificial Neural Networks – ICANN 2010 (ICANN 2010)

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Abstract

The application of the convolution neural network for detection of the micro nucleuses in the human lymphocyte images acquired by the image flow cytometer is considered in this paper. The existing method of detection, called IMAQ Match Pattern, is described and its limitations concerning zoom factors are analyzed. The training algorithm of the convolution neural network and the detection procedure were described. The performance of both detection methods, convolution neural network and IMAQ Match Pattern, were researched. Our results show that the convolution neural network overcomes the IMAQ Match Pattern in terms of improvement of detection rate and decreasing the numbers of false alarms.

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References

  1. Coskun, M., Top, A., Orta, T.: Biological Dosimetry Following X-ray Irradiation. Turkish Journal of Medical Science 30, 563–569 (2000)

    Google Scholar 

  2. Cram, L.S., Martin, J.C., Steinkamp, J.A., Yoshida, T.M., Buican, T.N., Marosiorone, B.L., Jett, J.H., Salzman, G., Sklar, L.: New Flow Cytometric Capabilities at the National Flow Cytometry Resource. Proc. of IEEE 80(6), 912–917 (1992)

    Article  Google Scholar 

  3. Liu, Y., Fisher, A.C.: Human Erythrocyte Sizing and Deformability Study by Laser Flow Cytometer. In: Proc. of Ann. Int. Conf. of the IEEE Eng. in Medicine and Biology Society, vol. 1, pp. 324–325 (1992)

    Google Scholar 

  4. Maguire, D., King, G.B., Kelley, S., Durack, G., Robinson, J.P.: Computer-Assisted Diagnosis of Hematological Malignancies Using a Pattern Representation of Flow Cytometry Data. In: Proc. of 12th South. Biom. Eng. Conf., vol. 1, pp. 153–155 (1993)

    Google Scholar 

  5. Abate, G.F., Bavaro, F., Castello, G., Daponte, P., Grimaldi, D., Guglielmelli, G., Martinelli, F.U., Mauro, U., Moisa, S., Napolitano, M., Rapuano, S., Scerbo, P.: Tomography System to Acquire 3D Images of Cells in Laminar Flow: Hardware Architecture. In: Proc. Intern. Workshop on Medical Measurement and Applications MeMea 2006, Italy, pp. 68–73 (2006)

    Google Scholar 

  6. Grimaldi, D., Lamonaca, F.: Reduction of Doubtful Detection of Micro-nucleus in Human Lymphocyte. Int. J. Advan. Media and Comm. 3(1/2), 80–94 (2009)

    Article  Google Scholar 

  7. Balestrieri, E., Grimaldi, D., Lamonaca, F., Rapuano, S.: Image Flow Cytometer. In: Murkopadhyay, S.C., Lay, E.A. (eds.) Adv. in Biomed. Sens., Meas., Instrum. and Syst. LNEE, vol. 55, pp. 210–239 (2010)

    Google Scholar 

  8. Relf, C.G.: Image Acquisition and Processing with LabVIEW, pp. 164–168. CRC Press, Boca Raton (2004)

    Google Scholar 

  9. Carnì, D.L., Grimaldi, D., Lamonaca, F.: Image Pre-processing for Micro Nucleuses Detection in Lymphocyte. Intern. Sci. J. of Computing 4(3), 63–69 (2005)

    Google Scholar 

  10. Carnì, D.L., Grimaldi, D., Lamonaca, F.: Pre-Processing Correction for Micro Nucleus Image Detection Affected by Contemporaneous Alterations. IEEE Transaction on I&M 56(4), 1202–1211 (2007)

    Google Scholar 

  11. Haykin, S.: Neural Networks and Learning Machines, 3rd edn. Prentice Hall, Englewood Cliffs (2008)

    Google Scholar 

  12. Yang, M.: Recent Advances in Face Detection. In: IEEE ICPR 2004, Tutorial, Cambridge, UK, 93 (2004)

    Google Scholar 

  13. Rowley, H., Baluja, S., Kanade, T.: Neural Network-based Face Detection. IEEE Trans. on Patt. Anal. and Mach. Intellig. 20, 22–88 (1998)

    Google Scholar 

  14. Garcia, C., Delakis, M.: Convolution Face Finder: A Neural Architecture for Fast and Robust Face Detection. IEEE Trans. on Pat. Anal. and Mach. Intellig. 26(11), 1408–1423 (2004)

    Article  Google Scholar 

  15. Romdhani, S., Torr, P., Schlkopf, B., Blake, A.: Computationally Efficient Face Detection. In: Proceedings of ICCV, vol. 1, pp. 695–700 (2001)

    Google Scholar 

  16. Heisele, B., Serre, T., Prentice, S., Poggio, T.: Hierarchical Classification and Feature Reduction for Fast Face Detection with Support Vector Machines. Pattern Recognition 36(9), 2007–2017 (2003)

    Article  MATH  Google Scholar 

  17. Schneiderman, H., Kanade, T.: Probabilistic Modeling of Local Appearance and Spatial Relationships for Object Recognition. In: Proc. IEEE Conf. Comp. Vision and Patt. Recog. pp. 45–51 (1998)

    Google Scholar 

  18. Viola, P., Jones, M.: Robust Real-Time Face Detection. Int. J. of Comp. Vis. 57(2), 137–154 (2004)

    Article  Google Scholar 

  19. Lienhart, R., Maydt, J.: An Extended Set of Haar-like Features for Rapid Object Detection. In: Proc. of IEEE Inter. Conf. on Image Proc. vol. 1, pp. 900–903 (2002)

    Google Scholar 

  20. Li, S., Zhang, Z.: FloatBoost Learning and Statistical Face Detection. IEEE Trans. on Patt. Analys. and Mach. Intellig. 26(9), 1112–1123 (2004)

    Article  Google Scholar 

  21. LeCun, Y., Bottou, L., Bengio, Y.: Gradient-Based Learning Applied to Document Recognition. Intellig. Sign. Proc., 306–351, IEEE Press (2001)

    Google Scholar 

  22. Simard, P., Steinkraus, D., Platt, J.: Best Practices for Convolutional Neural Networks Applied to Visual Document Analysis. In: 7th Intern. Conf. on Document Analys. and Recogn., vol. 2, p. 958 (2003)

    Google Scholar 

  23. Paliy, I.: Human Face Detection Methods Using a Combined Cascade of Classifiers, Inter. J. of Computing 7(1), 114–125 (2004) (in Ukrainian)

    Google Scholar 

  24. Wasserman, A.: Neural Computing: Theory and Practice, vol. 230. Van Nostrand Reinhold, New York (1989)

    Google Scholar 

  25. Golovko, V., Galushkin, A.: Neural Networks: Training, Models and Applications. Radiotechnika, Moscow, 256 (2001) (in Russian)

    Google Scholar 

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Paliy, I., Lamonaca, F., Turchenko, V., Grimaldi, D., Sachenko, A. (2010). Micro Nucleus Detection in Human Lymphocytes Using Convolutional Neural Network. In: Diamantaras, K., Duch, W., Iliadis, L.S. (eds) Artificial Neural Networks – ICANN 2010. ICANN 2010. Lecture Notes in Computer Science, vol 6352. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-15819-3_68

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  • DOI: https://doi.org/10.1007/978-3-642-15819-3_68

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-15818-6

  • Online ISBN: 978-3-642-15819-3

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