Abstract
Salmonella enterica serovar Typhi CT18 (S. Typhi) is the causative agent of typhoid fever in human beings. Currently, most of the drugs used to treat this sickness have adverse side-effects. Moreover, drug-resistant strains are emerging as a serious threat for the disease. Therefore, the most effective drug targets are urgently demanded for the development of new faster-acting antibacterial agents. In this paper, a published method for drug targets identification in Mycobacterium tuberculosis metabolismby Kalapanulak was applied to typhoid fever. The whole genome of S. Typhi was investigated and 282 genes were proposed as new drug targets. Interestingly, 34 drug–affected and essential genes from the three current antibiotics are all found in our proposed drug targets.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Kaye, K.S., Kaye, D.: Salmonella infections (including typhoid fever). In: Goldman, L., Ansiello, D. (eds.) Cecil Medicine, 23th edn. Saunders Elsevier, Philadelphia (2007)
Ivanoff, B., Levine, M.M.: Typhoid fever: continuing challenges from a resilient bacterial foe. Bulletin de l’Institut Pasteur 95, 129–142 (1997)
Shanahan, P.M., Jesudason, M.V., Thomson, C.J., Amyes, S.G.: Molecular analysis of and identification of antibiotic resistance genes in clinical isolates of Salmonella typhi from India. J. Clin. Microbiol. 36, 1595–1600 (1998)
Everest, P., et al.: The molecular mechanisms of severe typhoid fever. Trends in Microbiology 9, 316–320 (2001)
International Union of CRYSTALLOGRAPHY, http://ww1.iucr.org/people/wyckoff.htm
Ogata, H., et al.: KEGG: Kyoto Encyclopedia of Genes and Genomes. Nucleic Acids Res. 27, 29–34 (1999)
Apweiler, R., et al.: The Universal Protein Resource (UniProt) in 2010. Nucleic Acids Res. 38, D142–D148 (2010)
Hunter, S., et al.: InterPro: the integrative protein signature database. Nucleic Acids Res. 37, D211–D215 (2009)
Barh, D., Kumar, A.: In silico identification of candidate drug and vaccine targets from various pathways in Neisseria gonorrhoeae. Silico. Biol. 9, 225–231 (2009)
Dutta, A., et al.: In silico identification of potential therapeutic targets in the human pathogen Helicobacter pylori. In Silico. Biol. 6, 43–47 (2006)
Doyle, M.A., et al.: Drug target prediction and prioritization: using orthology to predict essentiality in parasite genomes. BMC Genomics 11, 1–14 (2010)
Kalapanulak, S.: High Quality Genome-Scale Metabolic Network Reconstruction of Mycobacterium tuberculosis and Comparison with Human Metabolic Network: Application for Drug Targets Identification. PhD Thesis. School of Informatics, The University of Edinburgh (2009)
Langridge, G.C., et al.: Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants. Genome Res. 19, 2308–2316 (2009)
Becker, D., et al.: Robust Salmonella metabolism limits possibilities for new antimicrobials. Nature 440, 303–307 (2006)
StatTrek Teach yourself statistics, http://www.StatTrek.com
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Juntrapirom, A., Kalapanulak, S., Saithong, T. (2010). Genome-Based Screening for Drug Targets Identification: Application to Typhoid Fever. In: Chan, J.H., Ong, YS., Cho, SB. (eds) Computational Systems-Biology and Bioinformatics. CSBio 2010. Communications in Computer and Information Science, vol 115. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-16750-8_19
Download citation
DOI: https://doi.org/10.1007/978-3-642-16750-8_19
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-16749-2
Online ISBN: 978-3-642-16750-8
eBook Packages: Computer ScienceComputer Science (R0)