Abstract
This study aims to investigate the correlation of PvuII (rs2234693) and XbaI (rs9340799) polymorphisms in estrogen receptor alpha (ESR1) gene with Chronic Hepatitis B Virus Infection, and provide new insight and scientific basis for controlling chronic infection of hepatitis B virus (HBV). We selected 107 healthy subjects as the control group and 107 patients with chronic hepatitis B (CHB) as the treated group. The molecular diagnosis methods of PCR-HRM based on the high-resolution melting curve (HRM) technique were employed to determine the gene polymorphisms of two SNPs: rs2234693 (T > C) and rs9340799 (A > G). The correlations between these two single nucleotide polymorphisms (SNP) sites and HBV chronic were further verified by gel electrophoresis and following sequencing. It was found that the genotype frequencies of rs2234693 (T > C) site were significantly different between CHB cases and the controls (P < 0.05), the allele frequency of rs2234693 showed no difference between two groups. The genotype frequencies of rs9340799 site was significantly different between CHB cases and the controls (P < 0.05). The GG genotype was significantly increased in the chronic hepatitis B group. The allele frequency of rs9340799 site was significantly different between the two groups (P < 0.05). According to Chi square analysis, the G gene of rs9340799 could increase the risk of chronic infection of HBV and the A gene could reduce the risk of chronic infection of HBV. The GG genotype and G allele of rs9340799 (A > G) of estrogen-receptor gene ESR1 may be the genetic susceptibility gene of HBV infection. The GG genotype is associated with chronic infection of HBV.
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Acknowledgment
This work was partially supported by National Natural Science Foundation of China (Grant No. 81773496, 2018PT01) and Natural Science Basic Research Plan in Shaanxi Province of China (Grant No. 2016JM8103, 2017PT22).
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Men, K. et al. (2020). Association of PvuII and XbaI Polymorphisms in Estrogen Receptor Alpha (ESR1) Gene with the Chronic Hepatitis B Virus Infection. In: Han, H., Wei, T., Liu, W., Han, F. (eds) Recent Advances in Data Science. IDMB 2019. Communications in Computer and Information Science, vol 1099. Springer, Singapore. https://doi.org/10.1007/978-981-15-8760-3_9
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