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Tetracycline Intelligent Target-Inducing Logic Gate Based on Triple-Stranded DNA Nanoswitch

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Book cover Bio-Inspired Computing: Theories and Applications (BIC-TA 2021)

Part of the book series: Communications in Computer and Information Science ((CCIS,volume 1565))

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Abstract

A previously unreported three-strand DNA (ts-DNA) non-metal structure with hairpin structure was designed based on a strategy of logic switching and tetracycline (TC) signaling molecule switching. Will target and TC-binding aptamer (TBA) is the combination of body, and its relation with three functional chain DNA conformation, with programmable DNA sensor signal by FAM input molecule fluorescence emission monitoring. After the combination, the input signal is converted to a fluorescent signal to perform a logical operation. By introducing a novel nanomaterial, graphene oxide (GO), as a signal regulator, the fluorescence response changed by intermolecular interactions is the key to achieving logical calculation. The harm of tetracycline can generally cause kidney damage, ear damage, gastrointestinal adverse reactions, skin allergies, etc.Using tetracycline as a molecular trigger to induce conformational changes in DNA, a logical operation target detection method was constructed. In addition, nanoscale devices can be reconfigured and optimized to accommodate additional logical calculations and target detection.

S. Xi and Y. Wang—These authors contributed equally to this work.

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Acknowledgment

This research was supported by National Natural Science Foundation of China (no. 61572302), Natural Science Basic Research Plan in Shaanxi Province of China (no. 2020JM-298). The authors are very grateful to the anonymous reviewers for their valuable comments and suggestions for improving the quality of the paper.

Funding

The data used to support the findings of this study are available from the corresponding author upon request.

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Authors and Affiliations

  1. College of Life Science, Shaanxi Normal University, Xi’an, 710119, Shaanxi, China

    Sunfan Xi, Luhui Wang, Meng Cheng & Yafei Dong

  2. School of Computer Science, Shaanxi Normal University, Xi’an, 710119, Shaanxi, China

    Yue Wang, Mengyang Hu & Yafei Dong

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  1. Sunfan Xi
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  2. Yue Wang
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  3. Mengyang Hu
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Corresponding author

Correspondence to Yafei Dong .

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Editors and Affiliations

  1. Huazhong University of Science and Technology, Wuhan, China

    Linqiang Pan

  2. Taiyuan University of Science and Technology, Taiyuan, China

    Zhihua Cui

  3. Taiyuan University of Science and Technology, Taiyuan, China

    Jianghui Cai

  4. Huazhong University of Science and Technology, Wuhan, China

    Lianghao Li

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Xi, S., Wang, Y., Hu, M., Wang, L., Cheng, M., Dong, Y. (2022). Tetracycline Intelligent Target-Inducing Logic Gate Based on Triple-Stranded DNA Nanoswitch. In: Pan, L., Cui, Z., Cai, J., Li, L. (eds) Bio-Inspired Computing: Theories and Applications. BIC-TA 2021. Communications in Computer and Information Science, vol 1565. Springer, Singapore. https://doi.org/10.1007/978-981-19-1256-6_30

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  • DOI: https://doi.org/10.1007/978-981-19-1256-6_30

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  • Publisher Name: Springer, Singapore

  • Print ISBN: 978-981-19-1255-9

  • Online ISBN: 978-981-19-1256-6

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