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Low level light therapy by Red–Green–Blue LEDs improves healing in an excision model of Sprague–Dawley rats

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Abstract

There are many methods of healing wounds. Among these, light therapy is reported to be beneficial, as beams assist the human body in treating, sterilizing, and regenerating cells. Both Laser and LED irradiation with specific wavelengths induce proliferation of fibroblasts depending on the wound area or wavelength and are effective in wound healing. This study used 8-week-old 250–300 g Male Sprague–Dawley Rats (ILAR Code: NTacSam:SD). The experiment was carried out for the non-irradiation group and the Red–Green–Blue LEDs irradiation groups (n of each group = 5). The experiment animals were relieved for 24 h after wounds had been excised and then the Red–Green–Blue LEDs irradiation groups were given irradiation therapy over 9 days 1 h per day. Immunohistochemical staining was conducted for cytokeratin in order to precisely measure the defect size. Also, Masson’s trichrome staining was conducted for comparison of collagen between the Red–Green–Blue LEDs irradiation groups and the non-irradiation group. Animals treated with Blue LEDs irradiation (p < 0.05), Green LEDs irradiation (p < 0.05), and Red LEDs irradiation (N.S.) healed at a faster rate than non-irradiated group. The LEDs irradiated groups also had more collagen, according to Masson’s trichrome staining for collagen analysis. The Red–Green–Blue LEDs irradiation had a beneficial effect on wound healing and could probably replace low power laser treatment.

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Authors and Affiliations

  1. Department of Biomedical Science, Dongshin University, 252 Daeho-dong, Naju, Jeonnam, 520-714, Republic of Korea

    Min Woo Cheon

  2. Department of Electrical and Electronic Engineering, Dongshin University, 252 Daeho-dong, Naju, Jeonnam, 520-714, Republic of Korea

    Tae Gon Kim

  3. Division of Computer Engineering, Mokwon University, Daejeon, 302-729, Republic of Korea

    Yang Sun Lee

  4. Department of Medicine, Chosun University, 375 Seosuk-dong, Dong-gu, Gwang-ju, 501-753, Republic of Korea

    Seong Hwan Kim

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  1. Min Woo Cheon
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  2. Tae Gon Kim
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  3. Yang Sun Lee
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Correspondence to Seong Hwan Kim.

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Cheon, M.W., Kim, T.G., Lee, Y.S. et al. Low level light therapy by Red–Green–Blue LEDs improves healing in an excision model of Sprague–Dawley rats. Pers Ubiquit Comput 17, 1421–1428 (2013). https://doi.org/10.1007/s00779-012-0577-3

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  • DOI: https://doi.org/10.1007/s00779-012-0577-3

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