Skip to main content

Advertisement

SpringerLink
Log in

A Three-dimensional Model of the Human Immunodeficiency Virus Type 1 Integration Complex

  • Published:
Journal of Computer-Aided Molecular Design Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Summary

While the general features of HIV-1 integrase function are understood, there is still uncertainty about the composition of the integration complex and how integrase interacts with viral and host DNA. We propose an improved model of the integration complex based on current experimental evidence including a comparison with the homologous Tn5 transposase containing bound DNA and an analysis of DNA binding sites using Goodford’s GRID. Our model comprises a pair of integrase dimers, two strands of DNA to represent the viral DNA ends and a strand of bent DNA representing the host chromosome. In our model, the terminal four base pairs of each of the viral DNA strands interact with the integrase dimer providing the active site, while bases one turn away interact with a flexible loop (residues 186–194) on the second integrase dimer. We propose that residues E152, Q148 and K156 are involved in the specific recognition of the conserved CA dinucleotide and that the active site mobile loop (residues 140–149) stabilises the integration complex by acting as a barrier to separate the two viral DNA ends. In addition, the residues responsible for DNA binding in our model show a high level of amino acid conservation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Brown, P.O., In Retroviruses, Coffin J.M., Hughes S.H. and Varmus H.E. (Eds.), Integration, Cold Spring harbour Press, Plainview, NY, 1997, 161 pp.

  2. P. Hindmarsh J. Leis (1999) Microbiol. Mol. Biol. Rev. 63 836 Occurrence Handle10585967

    PubMed  Google Scholar 

  3. S.A. Chow (1997) Methods 12 306 Occurrence Handle10.1006/meth.1997.0484 Occurrence Handle9245611

    Article  PubMed  Google Scholar 

  4. T.S. Heuer P.O. Brown (1998) Biochemistry 37 6667 Occurrence Handle10.1021/bi972949c Occurrence Handle9578550

    Article  PubMed  Google Scholar 

  5. K. Gao S.L. Butler F. Bushman (2001) EMBO J. 20 3565 Occurrence Handle10.1093/emboj/20.13.3565 Occurrence Handle11432843

    Article  PubMed  Google Scholar 

  6. J.Y. Wang H. Ling W. Yang R. Craigie (2001) EMBO J. 20 7333 Occurrence Handle10.1093/emboj/20.24.7333 Occurrence Handle11743009

    Article  PubMed  Google Scholar 

  7. A.A. Podtelezhnikov K. Gao F.D. Bushman J.A. McCammon (2003) Biopolymers 68 110 Occurrence Handle10.1002/bip.10217 Occurrence Handle12579583

    Article  PubMed  Google Scholar 

  8. Z.N. Yang T.C. Mueser F.D. Bushman C.C. Hyde (2000) J. Mol. Biol. 296 535 Occurrence Handle10.1006/jmbi.1999.3463 Occurrence Handle10669607

    Article  PubMed  Google Scholar 

  9. Z. Chen Y. Yan S. Munshi Y. Li J. Zugay-Murphy B. Xu M. Witmer P. Felock A. Wolfe V. Sardana E.A. Emini D. Hazuda L.C. Kuo (2000) J. Mol. Biol. 296 521 Occurrence Handle10.1006/jmbi.1999.3451 Occurrence Handle10669606

    Article  PubMed  Google Scholar 

  10. J.C. Chen J. Krucinski L.J. Miercke J.S. Finer-Moore A.H. Tang A.D. Leavitt R.M. Stroud (2000) Proc. Natl. Acad. Sci. USA. 97 8233 Occurrence Handle10.1073/pnas.150220297 Occurrence Handle10890912

    Article  PubMed  Google Scholar 

  11. A.L. Perryman J.A. McCammon (2002) J. Med. Chem. 45 5624 Occurrence Handle10.1021/jm025554m Occurrence Handle12477345

    Article  PubMed  Google Scholar 

  12. L. De Luca A. Pedretti G. Vistoli M. Letizia Barreca L. Villa P. Monforte A. Chimirri (2003) Biochem. Biophys. Res. Commun. 310 1083 Occurrence Handle10.1016/j.bbrc.2003.09.120 Occurrence Handle14559226

    Article  PubMed  Google Scholar 

  13. T.K. Chiu D.R. Davies (2004) Curr. Top. Med. Chem. 4 965 Occurrence Handle10.2174/1568026043388547 Occurrence Handle15134551

    Article  PubMed  Google Scholar 

  14. C. Marchand A.A. Johnson R.G. Karki G.C. Pais X. Zhang K. Cowansage T.A. Patel M.C. Nicklaus T.R. Burke SuffixJr. Y. Pommier (2003) Mol. Pharmacol. 64 600 Occurrence Handle10.1124/mol.64.3.600 Occurrence Handle12920196

    Article  PubMed  Google Scholar 

  15. Bushman, F.D., Fujiwara, T., Craigie, R., Science (Washington, DC), 249 (1990) 1555.

  16. F.D. Bushman R. Craigie (1991) Proc. Natl. Acad. Sci. USA. 88 1339 Occurrence Handle1847518

    PubMed  Google Scholar 

  17. A. Engelman K. Mizuuchi R. Craigie (1991) Cell 67 1211 Occurrence Handle10.1016/0092-8674(91)90297-C Occurrence Handle1760846

    Article  PubMed  Google Scholar 

  18. A. Acel B.E. Udashkin M.A. Wainberg E.A. Faust (1998) J. Virol. 72 2062 Occurrence Handle9499061

    PubMed  Google Scholar 

  19. E. Brin J. Yi A.M. Skalka J. Leis (2000) J.Biol. Chem. 275 39287 Occurrence Handle10.1074/jbc.M006929200 Occurrence Handle11006285

    Article  PubMed  Google Scholar 

  20. A. Engelman R. Craigie (1995) J. Virol. 69 5908 Occurrence Handle7637039

    PubMed  Google Scholar 

  21. M.S. Hansen G.J. Smith T. Kafri V. Molteni J.S. Siegel F.D. Bushman (1999) Nat. Biotechnol. 17 578 Occurrence Handle10.1038/9886 Occurrence Handle10385323

    Article  PubMed  Google Scholar 

  22. Y. Hwang D. Rhodes F. Bushman (2000) Nucl. Acids Res. 28 4884 Occurrence Handle10.1093/nar/28.24.4884 Occurrence Handle11121479

    Article  PubMed  Google Scholar 

  23. S.A. Chow K.A. Vincent V. Ellison P.O. Brown (1992) Science (Washington, D.C.) 255 723

    Google Scholar 

  24. G. Bujacz M. Jaskoski J. Alexandratos A. Wlodawer G. Merkel R.A. Katz A.M. Skalka (1995) J. Mol. Biol. 253 333 Occurrence Handle10.1006/jmbi.1995.0556 Occurrence Handle7563093

    Article  PubMed  Google Scholar 

  25. F. Dyda A.B. Hickman T.M. Jenkins A. Engelman R. Craigie D.R. Davies (1994) Science (Washington, D.C.) 266 1981

    Google Scholar 

  26. Y. Goldgur F. Dyda A.B. Hickman T.M. Jenkins R. Craigie D.R. Davies (1998) Proc. Natl. Acad. Sci. USA. 95 9150 Occurrence Handle10.1073/pnas.95.16.9150 Occurrence Handle9689049

    Article  PubMed  Google Scholar 

  27. Y. Goldgur R. Craigie G.H. Cohen T. Fujiwara T. Yoshinaga T. Fujishita H. Sugimoto T. Endo H. Murai D.R. Davies (1999) Proc. Natl. Acad. Sci. USA. 96 13040 Occurrence Handle10.1073/pnas.96.23.13040 Occurrence Handle10557269

    Article  PubMed  Google Scholar 

  28. J. Lubkowski F. Yang J. Alexandratos A. Wlodawer H. Zhao T.R. Burke SuffixJr. N. Neamati Y. Pommier G. Merkel A.M. Skalka (1998) Proc. Natl. Acad. Sci. USA. 95 4831 Occurrence Handle10.1073/pnas.95.9.4831 Occurrence Handle9560188

    Article  PubMed  Google Scholar 

  29. J. Lubkowski Z. Dauter F. Yang J. Alexandratos G. Merkel A.M. Skalka A. Wlodawer (1999) Biochemistry 38 13512 Occurrence Handle10.1021/bi991362q Occurrence Handle10521258

    Article  PubMed  Google Scholar 

  30. S. Maignan J.P. Guilloteau Q. Zhou-Liu C. Clement-Mella V. Mikol (1998) J. Mol. Biol. 282 359 Occurrence Handle10.1006/jmbi.1998.2002 Occurrence Handle9735293

    Article  PubMed  Google Scholar 

  31. P.J. Lodi J.A. Ernst J. Kuszewski A.B. Hickman A. Engelman R. Craigie G.M. Clore A.M. Gronenborn (1995) Biochemistry 34 9826 Occurrence Handle10.1021/bi00031a002 Occurrence Handle7632683

    Article  PubMed  Google Scholar 

  32. A. Eijkelenboom R. Sprangers K. Hard R.A.P. Lutzke R.H.A. Plasterk R. Boelens R. Kaptein (1999) Proteins: Struct., Funct., Genet. 36 556

    Google Scholar 

  33. A. Eijkelenboom F.M.I. Vandenent A. Vos J.F. Doreleijers K. Hard T.D. Tullius R.H.A. Plasterk R. Kaptein R. Boelens (1997) Curr. Biol. 7 739 Occurrence Handle10.1016/S0960-9822(06)00332-0 Occurrence Handle9368756

    Article  PubMed  Google Scholar 

  34. M.L. Cai R.L. Zheng M. Caffrey R. Craigie G.M. Clore A.M. Gronenborn (1997) Nat. Struct. Biol. 4 839 Occurrence Handle10.1038/nsb0797-567

    Article  Google Scholar 

  35. M.L. Cai Y. Huang M. Caffrey R.L. Zheng R. Craigie G.M. Clore A.M. Gronenborn (1998) Protein Sci. 7 2669 Occurrence Handle9865962

    PubMed  Google Scholar 

  36. T.S. Heuer P.O. Brown (1997) Biochemistry 36 10655 Occurrence Handle10.1021/bi970782h Occurrence Handle9271496

    Article  PubMed  Google Scholar 

  37. D. Esposito R. Craigie (1998) EMBO J. 17 5832 Occurrence Handle10.1093/emboj/17.19.5832 Occurrence Handle9755183

    Article  PubMed  Google Scholar 

  38. C. Vink A.M. Oude Groeneger R.H. Plasterk (1993) Nucl. Acids Res. 21 1419 Occurrence Handle8464733

    PubMed  Google Scholar 

  39. F.D. Bushman A. Engelman I. Palmer P. Wingfield R. Craigie (1993) Proc. Natl. Acad. Sci. USA. 90 3428 Occurrence Handle8386373

    PubMed  Google Scholar 

  40. R. Zheng T.M. Jenkins R. Craigie (1996) Proc. Natl. Acad. Sci. USA. 93 13659 Occurrence Handle10.1073/pnas.93.24.13659 Occurrence Handle8942990

    Article  PubMed  Google Scholar 

  41. F.M. Ent Particlevan den A. Vos R.H. Plasterk (1999) J. Virol. 73 3176 Occurrence Handle10074170

    PubMed  Google Scholar 

  42. V. Ellison J. Gerton K.A. Vincent P.O. Brown (1995) J. Biol. Chem. 270 3320 Occurrence Handle10.1074/jbc.270.7.3320 Occurrence Handle7852418

    Article  PubMed  Google Scholar 

  43. P.A. Rice T.A. Baker (2001) Nat. Struct. Biol. 8 302 Occurrence Handle10.1038/86166

    Article  Google Scholar 

  44. A. Engelman R. Craigie (1992) J. Virol. 66 6361 Occurrence Handle1404595

    PubMed  Google Scholar 

  45. A.D. Leavitt L. Shiue H.E. Varmus (1993) J. Biol. Chem. 268 2113 Occurrence Handle8420982

    PubMed  Google Scholar 

  46. D.C. Gent ParticleVan A.A.M. Oude Groeneger R.H.A. Plasterk (1992) Proc. Natl. Acad. Sci. USA. 89 9598 Occurrence Handle1409671

    PubMed  Google Scholar 

  47. P.M. Cannon W. Wilson E. Byles S.M. Kingsman A.J. Kingsman (1994) J. Virol. 68 4768 Occurrence Handle8035478

    PubMed  Google Scholar 

  48. A. Engelman G. Englund J.M. Orenstein M.A. Martin R. Craigie (1995) J. Virol. 69 2729 Occurrence Handle7535863

    PubMed  Google Scholar 

  49. A. Engelman A.B. Hickman R. Craigie (1994) J. Virol. 68 5911 Occurrence Handle8057470

    PubMed  Google Scholar 

  50. R.A. Lutzke C. Vink R.H. Plasterk (1994) Nucl. Acids Res. 22 4125 Occurrence Handle7937137

    PubMed  Google Scholar 

  51. S.R. Mumm D.P. Grandgenett (1991) J. Virol. 65 1160 Occurrence Handle1847445

    PubMed  Google Scholar 

  52. A. Eijkelenboom R.A.P. Lutzke R. Boelens R.H.A. Plasterk R. Kaptein K. Hard (1995) Nat. Struct. Biol. 2 807 Occurrence Handle10.1038/nsb0995-807 Occurrence Handle7552753

    Article  PubMed  Google Scholar 

  53. K. Zhu C. Dobard S.A. Chow (2004) J. Virol. 78 5045 Occurrence Handle10.1128/JVI.78.10.5045-5055.2004 Occurrence Handle15113886

    Article  PubMed  Google Scholar 

  54. E.A. Hehl P. Joshi G.V. Kalpana V.R. Prasad (2004) J. Virol. 78 5056 Occurrence Handle10.1128/JVI.78.10.5056-5067.2004 Occurrence Handle15113887

    Article  PubMed  Google Scholar 

  55. J.P.G. Mack J. Theochem (1998) Mol. Struct. 423 41

    Google Scholar 

  56. D.R. Davies L.M. Braam W.S. Reznikoff I. Rayment (1999) J. Biol. Chem. 274 11904 Occurrence Handle10.1074/jbc.274.17.11904 Occurrence Handle10207011

    Article  PubMed  Google Scholar 

  57. D.R. Davies I.Y. Goryshin W.S. Reznikoff I. Rayment (2000) Science (Washington, D.C.) 289 77

    Google Scholar 

  58. L. Haren B. Ton-Hoang M. Chandler (1999) Annu. Rev. Microbiol. 53 245 Occurrence Handle10.1146/annurev.micro.53.1.245 Occurrence Handle10547692

    Article  PubMed  Google Scholar 

  59. W.S. Reznikoff (2003) Mol. Microbiol. 47 1199 Occurrence Handle10.1046/j.1365-2958.2003.03382.x Occurrence Handle12603728

    Article  PubMed  Google Scholar 

  60. P. Rice R. Craigie D.R. Davies (1996) Curr. Opin. Struct. Biol. 6 76 Occurrence Handle10.1016/S0959-440X(96)80098-4 Occurrence Handle8696976

    Article  PubMed  Google Scholar 

  61. T.L. Williams T.A. Baker (2000) Science (Washington, D.C.) 289 73

    Google Scholar 

  62. T.M. Jenkins D. Esposito A. Engelman R. Craigie (1997) EMBO J. 16 6849 Occurrence Handle10.1093/emboj/16.22.6849 Occurrence Handle9362498

    Article  PubMed  Google Scholar 

  63. P.J. Goodford (1985) J. Med. Chem. 28 849 Occurrence Handle10.1021/jm00145a002 Occurrence Handle3892003

    Article  PubMed  Google Scholar 

  64. L. Holm C. Sander (1999) Nucl. Acids Res. 27 244 Occurrence Handle10.1093/nar/27.1.244 Occurrence Handle9847191

    Article  PubMed  Google Scholar 

  65. C. Kuiken B. Foley B. Hahn P. Marx F. McCutchan J. Mellors S. Wolinsky B. Korber (Eds) (2001) HIV Sequence Compendium 2001: Theoretical Biology and Biophysics Group Los Alamos National Laboratory Los Alamos, NM

    Google Scholar 

  66. D. Pruss F.D. Bushman A.P. Wolffe (1994) Proc. Natl. Acad. Sci. USA. 91 5913 Occurrence Handle8016088

    PubMed  Google Scholar 

  67. D. Pruss R. Reeves F.D. Bushman A.P. Wolffe (1994) J. Biol. Chem. 269 25031 Occurrence Handle7929189

    PubMed  Google Scholar 

  68. P.M. Pryciak H.E. Varmus (1992) Cell 69 769 Occurrence Handle10.1016/0092-8674(92)90289-O Occurrence Handle1317268

    Article  PubMed  Google Scholar 

  69. Y.C. Bor M.D. Miller F.D. Bushman L.E. Orgel (1996) Virology 222 283 Occurrence Handle10.1006/viro.1996.0422 Occurrence Handle8806511

    Article  PubMed  Google Scholar 

  70. R.A. Katz P. DiCandeloro G. Kukolj A.M. Skalka (2001) J. Biol. Chem. 276 34213 Occurrence Handle10.1074/jbc.M104632200 Occurrence Handle11441016

    Article  PubMed  Google Scholar 

  71. T.M. Jenkins A. Engelman R. Ghirlando R. Craigie (1996) J. Biol. Chem. 271 7712 Occurrence Handle10.1074/jbc.271.13.7712 Occurrence Handle8631811

    Article  PubMed  Google Scholar 

  72. M.D. Andrake A.M. Skalka (1995) J. Biol. Chem. 270 29299 Occurrence Handle10.1074/jbc.270.49.29299 Occurrence Handle7493962

    Article  PubMed  Google Scholar 

  73. E. Deprez P. Tauc H. Leh J.F. Mouscadet C. Auclair J.C. Brochon (2000) Biochemistry 39 9275 Occurrence Handle10.1021/bi000397j Occurrence Handle10924120

    Article  PubMed  Google Scholar 

  74. H. Leh P. Brodin J. Bischerour E. Deprez P. Tauc J.C. Brochon E. LeCam D. Coulaud C. Auclair J.F. Mouscadet (2000) Biochemistry 39 9285 Occurrence Handle10.1021/bi000398b Occurrence Handle10924121

    Article  PubMed  Google Scholar 

  75. E. Asante-Appiah A.M. Skalka (1999) Adv. Virus Res. 52 351 Occurrence Handle10384242

    PubMed  Google Scholar 

  76. E. Asante-Appiah A.M. Skalka (1997) J. Biol. Chem. 272 16196 Occurrence Handle10.1074/jbc.272.26.16196 Occurrence Handle9195919

    Article  PubMed  Google Scholar 

  77. C. Vink R.A. Lutzke R.H. Plasterk (1994) Nucl. Acids Res. 22 4103 Occurrence Handle7937134

    PubMed  Google Scholar 

  78. van Gent, D.C., Vink, C., Groeneger, A.A. and Plasterk, R.H., EMBO J., 12 (1993) 3261.

  79. Engelman, A., Bushman, F.D. and Craigie, R., EMBO J., 12 (1993) 3269.

  80. K.K. Bao H. Wang J.K. Miller D.A. Erie A.M. Skalka I. Wong (2003) J. Biol. Chem. 278 1323 Occurrence Handle10.1074/jbc.C200550200 Occurrence Handle12446721

    Article  PubMed  Google Scholar 

  81. W. Yang T.A. Steitz (1995) Structure 3 131 Occurrence Handle10.1016/S0969-2126(01)00142-3 Occurrence Handle7735828

    Article  PubMed  Google Scholar 

  82. E. Khan J.P. Mack R.A. Katz J. Kulkosky A.M. Skalka (1991) Nucl. Acids Res. 19 851 Occurrence Handle1850126

    PubMed  Google Scholar 

  83. K. Sayasith G. Sauve J. Yelle (2000) Mol. Cells 10 525 Occurrence Handle11101143

    PubMed  Google Scholar 

  84. A.M. Dirac J. Kjems (2001) Eur. J. Biochem. 268 743 Occurrence Handle10.1046/j.1432-1327.2001.01932.x Occurrence Handle11168414

    Article  PubMed  Google Scholar 

  85. M.L. Connolly (1983) Science (Washington, D.C.) 221 709

    Google Scholar 

  86. A. Engelman Y. Liu H.M. Chen M. Farzan F. Dyda (1997) J. Virol. 71 3507 Occurrence Handle9094622

    PubMed  Google Scholar 

  87. A.L. Harper L.M. Skinner M. Sudol M. Katzman (2001) J. Virol. 75 7756 Occurrence Handle10.1128/JVI.75.16.7756-7762.2001 Occurrence Handle11462051

    Article  PubMed  Google Scholar 

  88. R.S. Appa C.G. Shin P. Lee S.A. Chow (2001) J. Biol. Chem. 276 45848 Occurrence Handle10.1074/jbc.M107365200 Occurrence Handle11585830

    Article  PubMed  Google Scholar 

  89. K.A. Vincent V. Ellison S.A. Chow P.O. Brown (1993) J. Virol. 67 425 Occurrence Handle8416376

    PubMed  Google Scholar 

  90. S.A. Chow P.O. Brown (1994) J. Virol. 68 7869 Occurrence Handle7966577

    PubMed  Google Scholar 

  91. A. Engelman (1999) Adv. Virus Res. 52 411 Occurrence Handle10384245

    PubMed  Google Scholar 

  92. M. Wiskerchen M.A. Muesing (1995) J. Virol. 69 376 Occurrence Handle7983732

    PubMed  Google Scholar 

  93. J.L. Gerton S. Ohgi M. Olsen J. Derisi P.O. Brown (1998) J. Virol. 72 5046 Occurrence Handle9573274

    PubMed  Google Scholar 

  94. J. Greenwald V. Le S.L. Butler F.D. Bushman S. Choe (1999) Biochemistry 38 8892 Occurrence Handle10.1021/bi9907173 Occurrence Handle10413462

    Article  PubMed  Google Scholar 

  95. M.L. Barreca K.W. Lee A. Chimirri J.M. Briggs (2003) Biophys. J. 84 1450 Occurrence Handle12609852

    PubMed  Google Scholar 

  96. R.D. Lins J.M. Briggs T.P. Straatsma H.A. Carlson J. Greenwald S. Choe J.A. McCammon (1999) Biophys. J. 76 2999 Occurrence Handle10354426

    PubMed  Google Scholar 

  97. W. Weber H. Demirdjian R.D. Lins J.M. Briggs R. Ferreira J.A. McCammon (1998) J. Biomol. Struct. Dyn. 16 733 Occurrence Handle10052629

    PubMed  Google Scholar 

  98. H. Ni C.A. Sotriffer J.A. McCammon (2001) J. Med. Chem. 44 3043 Occurrence Handle10.1021/jm010205y Occurrence Handle11543671

    Article  PubMed  Google Scholar 

  99. Y. Pommier A.A. Pilon K. Bajaj A. Mazumder N. Neamati (1997) Antiviral Chem. Chemother. 8 463

    Google Scholar 

  100. F.M. Ent Particlevan den A. Vos R.H. Plasterk (1998) J. Virol. 72 3916 Occurrence Handle9557677

    PubMed  Google Scholar 

  101. K.M. McGrath N.G. Hoffman W. Resch J.A. Nelson R. Swanstrom (2001) Virus Res. 76 137 Occurrence Handle10.1016/S0168-1702(01)00271-4 Occurrence Handle11410314

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medicinal Chemistry, Monash University, 381 Royal Parade, 3052, Parkville, Vic., Australia

    Jerome Wielens, Ian T. Crosby & David K. Chalmers

Authors
  1. Jerome Wielens
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ian T. Crosby
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. David K. Chalmers
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jerome Wielens.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wielens, J., Crosby, I.T. & Chalmers, D.K. A Three-dimensional Model of the Human Immunodeficiency Virus Type 1 Integration Complex. J Comput Aided Mol Des 19, 301–317 (2005). https://doi.org/10.1007/s10822-005-5256-2

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10822-005-5256-2

Keywords

Search

Navigation