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Exocytotic dynamics in human chromaffin cells: experiments and modeling

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Abstract

Chromaffin cells have been widely used to study neurosecretion since they exhibit similar calcium dependence of several exocytotic steps as synaptic terminals do, but having the enormous advantage of being neither as small or fast as neurons, nor as slow as endocrine cells. In the present study, secretion associated to experimental measurements of the exocytotic dynamics in human chromaffin cells of the adrenal gland was simulated by using a model that combines stochastic and deterministic approaches for short and longer depolarizing pulses, respectively. Experimental data were recorded from human chromaffin cells, obtained from healthy organ donors, using the perforated patch configuration of the patch-clamp technique. We have found that in human chromaffin cells, secretion would be mainly managed by small pools of non-equally fusion competent vesicles, slowly refilled over time. Fast secretion evoked by brief pulses can be predicted only when 75% of one of these pools (the “ready releasable pool” of vesicles, abbreviated as RRP) are co-localized to Ca2 +  channels, indicating an immediately releasable pool in the range reported for isolated cells of bovine and rat (Álvarez and Marengo, J Neurochem 116:155–163, 2011). The need for spatial correlation and close proximity of vesicles to Ca2 +  channels suggests that in human chromaffin cells there is a tight control of those releasable vesicles available for fast secretion.

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Acknowledgements

The authors thank Prof. Luis Miguel Gutiérrez (Instituto de Neurociencias, Alicante) for useful comments. AG, VGV and JS acknowledge I-MATH (project C3-0136) financial support. VGV acknowledges financial support from CONACyT-México. AHV holds a fellowship award from the Universidad Autónoma de Madrid. This work was supported by a grant from the Ministerio de Ciencia y Tecnología No. BFU2008-01382/BFI and BFU2011-27690 awarded to AA.

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Authors and Affiliations

  1. Departamento de Farmacología y Terapéutica, Facultad de Medicina, Universidad Autónoma de Madrid, 28029, Madrid, Spain

    Almudena Albillos, Alberto Pérez-Álvarez, Alicia Hernández-Vivanco & José Carlos Caba-González

  2. Depto. de Matemática Aplicada y Ciencias de la Computación, Universidad de Cantabria, 39005, Santander, Spain

    Amparo Gil

  3. Area de Química Aplicada, Universidad Autónoma Metropolitana Azcapotzalco, 02200, México, D.F., Mexico

    Virginia González-Vélez

  4. Depto. de Matemáticas, Estadística y Computación, Universidad de Cantabria, 39005, Santander, Spain

    Javier Segura

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  1. Almudena Albillos
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  2. Amparo Gil
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  3. Virginia González-Vélez
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  4. Alberto Pérez-Álvarez
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  5. Javier Segura
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  6. Alicia Hernández-Vivanco
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  7. José Carlos Caba-González
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Correspondence to Amparo Gil.

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Action Editor: Erik De Schutter

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Albillos, A., Gil, A., González-Vélez, V. et al. Exocytotic dynamics in human chromaffin cells: experiments and modeling. J Comput Neurosci 34, 27–37 (2013). https://doi.org/10.1007/s10827-012-0404-x

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  • DOI: https://doi.org/10.1007/s10827-012-0404-x

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