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Construction of a genetic conditional learning system in Escherichia coli

大肠杆菌中条件学习系统构建

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Abstract

Currently, there are a lot of man-made biomolecular computing systems. However, it is rare to see man-made biomolecular intelligence system. Learning ability is one of the basic abilities of intelligence. Many organisms have learning ability naturally. In this paper, we have successfully designed, constructed, and tested a genetic conditional learning system in the bacterium Escherichia coli. The system can recognize ‘bad guy’ signal with the help of ‘study’ signal. The function was realized by combining biomolecular AND gate and memory inverter. The AND gate used riboswitch as computation tool, and the memory inverter used Cre recombinase. Flow cytometry was used to verify the function of the system. This study is an attempt of constructing manmade biomolecular intelligence system. The system may lead to new applications for biomolecular computing, artificial intelligence, and biotherapy.

摘要

本文通过联用与门和记忆反相器两种生物分子计算部件, 在大肠杆菌内构建了一个条件学习系统。该系统能在“学习”信号的帮助下认识“坏人”。初始状态下, 该系统会欢迎“坏人”( 发出黄色荧光) ; 当“学习”信号和“坏人”同时出现时, 系统产生记忆; 之后, 系统不再欢迎“坏人”。本项研究是人工构建生物分子智能系统的一次有益尝试。

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Authors and Affiliations

  1. School of Information Engineering, Minzu University of China, Beijing, 100081, China

    Mei Chen

  2. Institute of Software, School of Electronics Engineering and Computer Science, Key Laboratory for High Confidence Software Technologies, Ministry of Education, Peking University, Beijing, 100871, China

    Mei Chen & Jin Xu

Authors
  1. Mei Chen
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  2. Jin Xu
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Correspondence to Mei Chen or Jin Xu.

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Chen, M., Xu, J. Construction of a genetic conditional learning system in Escherichia coli . Sci. China Inf. Sci. 58, 1–6 (2015). https://doi.org/10.1007/s11432-015-5308-8

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  • DOI: https://doi.org/10.1007/s11432-015-5308-8

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