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A low noise remotely controllable wireless telemetry system for single-unit recording in rats navigating in a vertical maze

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Abstract

The use of cables for recording neural activity limits the scope of behavioral tests used in conscious free-moving animals. Particularly, cable attachments make it impossible to record in three-dimensional (3D) mazes where levels are vertically stacked or in enclosed spaces. Such environments are of particular interest in investigations of hippocampal place cells, in which neural activity is correlated with spatial position in the environment. We developed a flexible miniaturized Bluetooth-based wireless data acquisition system. The wireless module included an 8-channel analogue front end, digital controller, and Bluetooth transceiver mounted on a backpack. Our bidirectional wireless design allowed all data channels to be previewed at 1 kHz sample rate, and one channel, selected by remote control, to be sampled at 10 kHz. Extracellular recordings of neuronal activity are highly susceptible to ambient electrical noise due to the high electrode impedance. Through careful design of appropriate shielding and hardware configuration to avoid ground loops, mains power and Bluetooth hopping frequency noise were reduced sufficiently to yield signal quality comparable to those recorded by wired systems. With this system we were able to obtain single-unit recordings of hippocampal place cells in rats running an enclosed vertical maze, over a range of 5 m.

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References

  1. Barrett CJ, Guild SJ, Ramchandra R et al (2005) Baroreceptor denervation prevents sympathoinhibition during angiotensin II-induced hypertension. Hypertension 46:168–172

    Article  Google Scholar 

  2. Brun VH, Otnass MK, Molden S et al (2002) Place cells and place recognition maintained by direct entorhinal-hippocampal circuitry. Science 296:2243–2246

    Article  Google Scholar 

  3. Chen HY, Wu JS, Li CP et al (2008) Wireless telemetry system for single-unit recording in rats navigation. J Chin Inst Eng 31:449–458

    Google Scholar 

  4. Fenton AA, Muller RU (1998) Place cell discharge is extremely variable during individual passes of the rat through the firing field. Proc Natl Acad Sci USA 95:3128

    Article  Google Scholar 

  5. Fox SE, Ranck JBJ (1981) Electrophysiological characteristics of hippocampal complex-spike cells and theta cells. Exp Brain Res 41:399–410

    Article  Google Scholar 

  6. Hawley ES, Hargreaves EL, Kubie JL et al (2002) Telemetry system for reliable recording of action potentials from freely moving rats. Hippocampus 12:505–513

    Article  Google Scholar 

  7. Jeffery KJ, Anand RL, Anderson MI (2006) A role for terrain slope in orienting hippocampal place fields. Exp Brain Res 169:218–225

    Article  Google Scholar 

  8. Jeffery KJ, Anderson MI, Hayman R, Chakraborty S (2004) A proposed architecture for the neural representation of spatial context. Neurosci Biobehav Rev 28:201–218

    Article  Google Scholar 

  9. Knierim JJ, McNaughton BL (2001) Hippocampal place-cell firing during movement in three-dimensional space. J Neurophysiol 85:105–116

    Google Scholar 

  10. Lenck-Santini PP, Save E, Poucet B (2001) Evidence for a relationship between place-cell spatial firing and spatial memory performance. Hippocampus 11:377–390

    Article  Google Scholar 

  11. O’keefe J, Dostrovsky J (1971) The hippocampus as a spatial map Preliminary evidence for unit activity in the freely-moving rat. Brain Res 34:171–175

    Article  Google Scholar 

  12. Obeid I, Nicolelis MA, Wolf PD (2004) A multichannel telemetry system for single unit neural recordings. J Neurosci Methods 133:33–38

    Article  Google Scholar 

  13. Shapiro ML, Tanila H, Eichenbaum H (1997) Cues that hippocampal place cells encode: dynamic and hierarchical representation of local and distal stimuli. Hippocampus 7:624–642

    Article  Google Scholar 

  14. Uemura K, Kawada T, Sugimachi M et al (2004) A self-calibrating telemetry system for measurement of ventricular pressure-volume relations in conscious, freely moving rats. Am J Physiol Heart Circ Physiol 287:H2906–H2913

    Article  Google Scholar 

Download references

Acknowledgments

This project was partially supported by grants from the National Science Council, Taiwan, R.O·C. (NSC93-2213-E-006-046) and from the National Health Research Institute of R.O·C. under contract no. NHRI-EX 95-9524E1.

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Authors and Affiliations

  1. Institute of Biomedical Engineering, National Cheng Kung University, 701 No. 1 Ta-Hsueh Road, Tainan, Taiwan, ROC

    Hsin-Yung Chen & Jia Jin Jason Chen

  2. Department of Family Medicine, Medical College, National Cheng Kung University, Tainan, Taiwan, ROC

    Jin-Shang Wu

  3. Department of Physiology, University of Otago, Dunedin, New Zealand

    Brian Hyland & Xiao-Dong Lu

Authors
  1. Hsin-Yung Chen
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  2. Jin-Shang Wu
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  3. Brian Hyland
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  4. Xiao-Dong Lu
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  5. Jia Jin Jason Chen
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Correspondence to Jia Jin Jason Chen.

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Chen, HY., Wu, JS., Hyland, B. et al. A low noise remotely controllable wireless telemetry system for single-unit recording in rats navigating in a vertical maze. Med Biol Eng Comput 46, 833–839 (2008). https://doi.org/10.1007/s11517-008-0355-6

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  • DOI: https://doi.org/10.1007/s11517-008-0355-6

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