Electron Transfer Mediated Biosensor with Plasma-Polymerized Film Containing Redox Site

Hitoshi MUGURUMA
Hideyuki UEHARA

Publication
IEICE TRANSACTIONS on Electronics   Vol.E89-C    No.12    pp.1781-1785
Publication Date: 2006/12/01
Online ISSN: 1745-1353
DOI: 10.1093/ietele/e89-c.12.1781
Print ISSN: 0916-8516
Type of Manuscript: Special Section PAPER (Special Section on Towards the Realization of Organic Molecular Electronics)
Category: Bioelectronics and Sensors
Keyword: 
glucose biosensor,  electron transfer mediator,  plasma polymerization,  ferrocene,  enzyme,  

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Summary: 
An electron transfer mediated amperometric enzyme biosensor based on a plasma-polymerized thin film of dimethylaminomethylferrocene (DMAMFc) is reported. A nanoscale thin polymer film containing a redox mediator was plasma-deposited directly onto an electrode with physisorbed glucose oxidase (GOD). Since the redox sites were introduced in the vicinity of the reaction centers of GOD, a highly efficient electron transfer system was formed in which almost all the reaction centers of GOD were connected to redox sites of the polymer matrix. The advantages of this strategy were: no need for prior or further chemical modification of the enzyme molecules, and simplicity of design compared with the use of a conventional polymer matrix. Moreover, the fact that the film deposition was performed using a microfabrication-compatible organic plasma promised great potential for high-throughput production of bioelectronic devices.