Adsorption of Antibody Protein onto Plasma-Polymerized Film Characterized by Atomic Force Microscopy and Quartz Crystal Microbalance

Hitoshi MUGURUMA
Satoshi MIURA
Naoya MURATA

Publication
IEICE TRANSACTIONS on Electronics   Vol.E90-C    No.3    pp.649-651
Publication Date: 2007/03/01
Online ISSN: 1745-1353
DOI: 10.1093/ietele/e90-c.3.649
Print ISSN: 0916-8516
Type of Manuscript: LETTER
Category: Organic Molecular Electronics
Keyword: 
antibody,  plasma-polymerized film,  adsorption,  

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Summary: 
Adsorption of antibody protein (anti-human IgG) onto plasma-polymerized thin films (PPF) with nanoscale thickness was characterized by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). The PPF surface is very flat (less than 1 nm roughness) and its properties (charge and wettability) can be easily changed while retaining the backbone structure. We focus on two types of surfaces: one is the pristine surface of hexamethyldisiloxane (HMDS) PPF (hydrophobic) and the other is an HMDS PPF surface with nitrogen-plasma treatment (hydrophilic and positive-charged surface). The AFM image showed that the antibody molecules were densely adsorbed onto both surfaces and individual antibody molecules could be observed. The QCM profiles show a corresponding tendency with the AFM images. These results indicate that the plasma polymerized film can be the suitable biointerface for the application of biosensor and bioassay.