Detection of Label-Free T4-DNA Molecules Using SPR Technique

Hiroki OKUNO
Ayami NISHIOKA
Maho HOSOGI
Fumikazu OOHIRA
Gen HASHIGUCHI

Publication
IEICE TRANSACTIONS on Electronics   Vol.E90-C    No.1    pp.110-115
Publication Date: 2007/01/01
Online ISSN: 1745-1353
DOI: 10.1093/ietele/e90-c.1.110
Print ISSN: 0916-8516
Type of Manuscript: Special Section PAPER (Special Section on Microoptomechatronics)
Category: Plasmonics and Nanophotonics
Keyword: 
DNA,  label free,  surface plasmon resonance,  SPR,  

Full Text: PDF(1.5MB)>>
Buy this Article


Summary: 
In this paper, we propose a new method for detecting label-free T4-DNA molecules quantitatively using a surface plasmon resonance (SPR) technique on a gold thin film. We used a solution that dissolved T4-DNA molecules in pure water, and examined the relationship between DNA concentration change and SPR angle change in the solution. As a result, it was confirmed that the SPR angle change increased with increasing DNA concentration change. Therefore, it was feasible to detect the DNA concentration change using the SPR technique. Furthermore, to examine and detect a single or a few DNA molecule, we tried to fabricate an SPR chip in which SPR area is narrowed so that it has the same effect as focusing the beam. To narrow the SPR area, we decreased the area of gold thin film in this chip, and, to reflect light from only the area of gold thin film, the area without a gold thin film was micromachined to increase its unevenness for the reduction of light reflection. By the above-mentioned method, we examined the possibility of detecting a label-free DNA molecule using the SPR technique.