The method does not require any assumption about the shapes of the components curves. It is, however, assumed that the components are nonnegative. The method requires normalization of component curves. The sum of squares of each component curve to be estimated is normalized so as to be unity. A first-pass cardiac study and a hepatic study are selected for investigation of applicability of the method. The method is investigated numerically using computer-generated time-activity curves with six components for the cardiac study and seven components for the hepatic study. Within the regions studied, the results demonstrate that the method can recover the component time-activity curves and the corresponding images of physiological components from a sequence of dynamic radionuclide images. The ability of the method to recover physiological components and its limitations are discussed, and potential applications are suggested.<>