Temperature is a very important factor in laser moxibustion. This paper foucus on temperature field in simulated living-porcine liver tissue radiated by laser. Firstly, we place simulated blood vessels in isolated porcine liver to simulate living liver and obtain simulation temperature field by the finite element analysis; then we use K type thermocouples and thermocouple amplifier respectively measured the temperature field of isolated porcine liver and simulated living liver. The experiment results of the two are basically consistent, in the same position of organization, blood perfusion rate will be significantly lower the temperature. These researches provide the basis for the establishment of laser moxibustion in TCM.