This paper presents the design of a high-voltage (HV) rail-to-rail error amplifier. This circuit controls the output signal of a low drop-out voltage regulator (LDO) according to the reference voltages and based on stacked standard transistors. The circuit is designed using 65 nm CMOS process technology with a nominal voltage of 2.5 V and is optimized for arbitrary values of supply voltage up to 5.0 V. The error amplifier consists of 3 stages and 2 feedback loops. The stages are internally connected rail-to-rail to achieve high GBW and DC accuracy. The simulation and measurement results for the designed HV-error amplifier show that the output signal of the LDO tracks the reference voltages. The circuit design is technology-independent and compatible with scaled CMOS.