Sub-pixel interpolation is one of the most computationally intensive parts of High Efficiency Video Coding (HEVC) video encoder and decoder. High-level synthesis (HLS) tools are started to be successfully used for FPGA implementations of digital signal processing algorithms. Therefore, in this paper, the first FPGA implementation of HEVC sub-pixel (half-pixel and quarter-pixel) interpolation algorithm using a HLS tool in the literature is proposed. The proposed HEVC sub-pixel interpolation hardware is implemented on Xilinx FPGAs using Xilinx Vivado HLS tool. It, in the worst case, can process 45 quad full HD (3840×2160) video frames per second. Using HLS tool significantly reduced the FPGA development time. Therefore, HLS tools can be used for FPGA implementation of HEVC video encoder.