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Magnetic resonance spectroscopy and imaging can differentiate between engineered bone and engineered cartilage | IEEE Conference Publication | IEEE Xplore

Magnetic resonance spectroscopy and imaging can differentiate between engineered bone and engineered cartilage


Abstract:

In the situation when both cartilage and its underlying bone are damaged, osteochondral tissue engineering is being developed to provide a solution. In such cases, the ab...Show More

Abstract:

In the situation when both cartilage and its underlying bone are damaged, osteochondral tissue engineering is being developed to provide a solution. In such cases, the ability to non-invasively monitor and differentiate the development of both cartilage and bone tissues is important. Nuclear magnetic resonance (NMR) spectroscopy and magnetic resonance imaging (MRI) have been widely used to non-invasively assess tissue-engineered cartilage and tissue-engineered bone. The purpose of this work is to assess differences in MR properties of tissue-engineered bone and tissue-engineered cartilage generated from the same cell-plus-scaffold combination at the early stage of tissue growth. We developed cartilage and bone tissue constructs by seeding human marrow stromal cells (HMSCs, 2 million/ml) in 1:1 collagen/chitosan gel for four weeks. The chondrogenic or osteogenic differentiation of cells was directed with the aid of a culture medium containing chondrogenic or osteogenic growth factors, respectively. The proton and sodium NMR and waterproton T1, T2 and diffusion MRI experiments were performed on these constructs and the control collagen/chitosan gel using a 9.4 T (1H freq. = 400 MHz) and a 11.7 T (1H freq. = 500 MHz) NMR spectrometers. In all cases, the development of bone and cartilage was found to be clearly distinguishable using NMR and MRI. We conclude that MRS and MRI are powerful tools to assess growing osteochondral tissue regeneration.
Date of Conference: 26-30 August 2014
Date Added to IEEE Xplore: 06 November 2014
Electronic ISBN:978-1-4244-7929-0

ISSN Information:

PubMed ID: 25570851
Conference Location: Chicago, IL, USA

References

References is not available for this document.