Cells sense external mechanical stimulus and respond to it through mechanotransduction mechanism. Fluid shear stress (FSS) has been found to be an important element among the mechanical stimuli. Recent advancements in microfluidics made mechanotransduction studies possible in near physiological conditions using microfluidic devices. FSS on human cells covers a broad range from very low level experienced due to interstitial flows (0.1 mPa) to very high level in aorta (10 Pa). In the present communication, we have designed a novel microfluidic device which can generate FSS on cells of five different orders with single inflow of fluid which can cover the whole range of physiological fluid shear stresses in one run. The dimensions of the device were calculated taking a resistance model for the micro channels. Flow velocities and wall shear stress were predicted through computer simulation. Shear stress values were analyzed for two different depths of channels and different inlet flow rates ranging from 50 to 0.5 μl/s. FSS was found to increase linearly with inlet flow rate and the stress profile was flatter for lesser depth of channel.