The application of biomedical imaging and image processing to radiation therapy with accelerated particles has unique challenges. The potential of particle therapy to precisely tailor the dose distribution around the target volume needs to account for the intrinsic sensitivity to uncertainties in dose deposition. These peculiar features motivate the use of image guided methods to consistently verify the accuracy in dose delivery. Dedicated imaging and image processing methods are required, from treatment planning to treatment verification phases, in order to reduce the effects of uncertainties. The scenario is also complicated by the lack of standardized layouts of treatment bunkers, which implies the relatively increased use of custom solutions. Conversely, imaging can be applied to verify the actual delivered dose, representing a valuable opportunity to validate specific protocols and visualize the efficacy of the intended treatment. In this contribution, challenges and opportunities in image guided particle therapy are overviewed, with a clear focus on research perspectives in biomedical imaging and image processing.