Understanding the effect of exogenous substances on human skin is critical for toxicology assessment. To address this, numerous artificial models of the topmost layer of human skin, so-called reconstructed human epidermis (RhE), have been created in an attempt to produce a clear analogue for testing. Unfortunately, current testing modalities still rely on endpoint assays and are not capable of monitoring time-resolved changes in barrier function without using numerous redundant samples. In this work, a novel, time-resolved approach is realized by monitoring the transepithelial electrical resistance (TEER) of MatTek EpiDerm® reconstructed human epidermis model, utilizing an automated protocol with the Intelligent Mobile Lab for in vitro diagnostics (IMOLA-IVD).