We demonstrate the biopsy needle capable of multi-spot impedance sensing based on attachable and flexible sensor film. In order to directly integrate sensor electrodes into curved surface of biopsy needle, attachable and thin polyimide substrate was used. Sensor electrodes were easily manipulated due to advantage of conventional microfabrication technique and this enable capability of multi-spot impedance sensing. To verify validity of proposed method, attachability of sensor film and real-time response of multi-spot sensing of fabricated biopsy needle was investigated.