This paper describes a new alternative to conventional radiography system currently used for radiostereometric analysis studies. Instead of using two non-calibrated X-ray sources with a cumbersome calibration cage, we propose to use the biplanar radiography EOS system. Its fixed configuration provides a preliminary calibration and a much simpler acquisition protocol. A flexible and accurate calibration method is presented to optimize EOS default calibration using a simple object and a self-calibration method. To validate our system, we calculate the 3D reconstruction error of a known object. Results showed an accuracy of 70±11μm and 0.05±0.02° for translation and rotation respectively, and an average epipolar error of 23±03μm.