This paper proposes an image matching system using aerial images, captured in flight time, and aerial geo-referenced images to estimate the Unmanned Aerial Vehicle (UAV) position in a situation of Global Navigation Satellite System (GNSS) failure. The image matching system is based on edge detection in the aerial and geo-referenced image and posterior automatic image registration of these edge-images (position estimation of UAV). The edge detection process is performed by an Artificial Neural Network (ANN), with an optimal architecture. A comparison with Sobel and Canny edge extraction filters is also provided. The automatic image registration is obtained by a cross-correlation process. The ANN optimal architecture is set by the Multiple Particle Collision Algorithm (MPCA). The image matching system was implemented in a low cost/consumption portable computer. The image matching system has been tested on real flight-test data and encouraging results have been obtained. Results using real flight-test data will be presented.