Nowadays single cell analysis becomes a more and more important method to gather the information of individual cells and study the heterogeneity of cells caused by random expression of gene, protein and the level of metabolism. Many device and technologies of single cell analysis have been developed to meet these needs. In this paper, we presented a high-throughput microchannel for single cell immobilization with small sheer pressure. It features high density arrays, which can accommodate up to 130~300 traps within 1~2 mm². According to our experiment, about 91% of capture unit can be occupied by the single cell in 40 s, using the optimized structure of microchannels. Therefore, we expect that the high throughput microchannels can be of great importance for the biological research.