Modeling and base-calling for Dna Sequencing-By-Synthesis | IEEE Conference Publication | IEEE Xplore

Modeling and base-calling for Dna Sequencing-By-Synthesis


Abstract:

The process of DNA sequencing-by-synthesis and its non-idealities are modeled as a noisy switched linear system parameterized by the unknown DNA sequence. The base-callin...Show More

Abstract:

The process of DNA sequencing-by-synthesis and its non-idealities are modeled as a noisy switched linear system parameterized by the unknown DNA sequence. The base-calling problem is then formulated as a parameter detection problem. As this system can have long memory, performing exact maximum-likelihood decoding is computationally prohibitive. An approximate ML method applied to experimental Pyrosequencing data demonstrates reliable read lengths exceeding 200 bases, which is significantly longer than that achieved by current methods
Date of Conference: 14-19 May 2006
Date Added to IEEE Xplore: 24 July 2006
Print ISBN:1-4244-0469-X

ISSN Information:

Conference Location: Toulouse, France

References

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