In this document, a new and efficient method to specify the ground truth locations of lane markers is presented. The method comprises of a novel process called Time-Slicing that provided the user with a unique visualization of the video. Coupled with automation via spline interpolation, the quick generation of necessary ground truth information is achieved. Videos recorded from a vehicle while driving on local city roads and highways are marked with ground truth information for use in testing. The performance of a variety of lane detection systems is compared to the ground truth and the error is computed for each system. Finally, quantitative analysis shows that the reference lane detection system presented in produces the most accurate lane detections which is depicted by the smallest error.