In this paper, we propose a new supervised monaural source separation based on autoencoders. We employ the autoencoder for the dictionary training such that the nonlinear network can encode the target source with high expressiveness. The dictionary is trained by each target source without the mixture signal, which makes the system independent from the context where the dictionaries will be used. In separation process, the decoder portions of the trained autoencoders are used as dictionaries to find the activations in a iterative manner such that a summation of the decoder outputs approximates the original mixture. The results of the instruments source separation experiments revealed that the separation performance of the proposed method was superior to that of the NMF.