In this paper, a cylindrical dielectric resonator antenna (DRA) with high isolation is investigated for MIMO applications. High isolation is achieved with the help of two different ports. The MIMO antenna consists of a cylindrical dielectric resonator antenna which is fed by two differently polarized feed lines for orthogonal mode excitation. The impedance bandwidth for port 1 and port 2 is 12.8 %(3.17–3.6 GHz) and 14.09 % (3.1–3.57 GHz) respectively. The isolation between the two ports is better than −27dB in the operating frequency band. The presented antenna is examined by calculating various MIMO performance parameter such as envelope correlation coefficient (ECC), diversity gain (DG), mean effective gain (MEG) by using S-parameter. The proposed antenna is suitable for WiMax applications.