As we all know that the precision of data, which get from the measure part of the control system in modern industry, makes obvious influence in the whole system. High precision is always the key issue in the target recognition and location. The image measurement technology is one of the important branches. Depend on the research a new subpixel location method based on genetic algorithm (GA) is proposed. The principle and implementation steps of the improved method are analyzed. On the basis of the analysis of this method, several experiments are given. The experimental results of the simulation are discussed which proved the feasibility and effectiveness of the scheme.