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Investigation into the use of electrochemical impedance spectroscopy for cellular functional immunophenotyping | IEEE Conference Publication | IEEE Xplore

Investigation into the use of electrochemical impedance spectroscopy for cellular functional immunophenotyping


Abstract:

We present here work towards the development of a lab-on-a-chip device used in the quantification of secreted cytokines from a small population of captured immune cells. ...Show More

Abstract:

We present here work towards the development of a lab-on-a-chip device used in the quantification of secreted cytokines from a small population of captured immune cells. This is accomplished by integrating microfluidic technology with interdigitated microelectrodes, which are used to quantify proteins using electrochemical impedance spectroscopy. Presented in detail is the effect of several parameters on the performance of the electrochemical biosensors, and a brief discussion of the microfluidic technology integrated with these biosensors in order to create a platform for cellular immunophenotyping. The developed sensor had a dynamic range from 0.1 ng/mL to 100 ng/mL, and the detection sensitivities at various frequencies are noted. It was also found that a redox probe concentration of 2mM is optimum for EIS measurements.
Published in: 2016 IEEE SENSORS
Date of Conference: 30 October 2016 - 03 November 2016
Date Added to IEEE Xplore: 09 January 2017
ISBN Information:
Conference Location: Orlando, FL, USA

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