The world is facing the pandemic of infectious diseases. Together with the development of transportation technology, the global spread of diseases continues to accelerate. To examine the correlation between transportation and infection, we did the statistical analysis on the highway transport and the domestic flight based on the GIS in the South Korea. Consequentially, the traffic movement through the domestic flight not only through expressway had more relevance to infection of diseases. By performing the SEIR simulation using Anylogic, the regional infection can be predicted when outbreaks occur to make quick responses of threats of diseases.