Although virtual reality technology is becoming more mature, there are still mutual constraints in the input and output methods of human-computer interaction, and the virtual hand-based interaction method will impose constraints on its interface layout. This paper aims to explore and verify the effectiveness of standardized 3D interface layouts to meet the needs of virtual hand interaction and conducts a comparative analysis of various 3D interface layouts. Taking an interface optimization case of a generalized daily 3D application as a practice, the interface layout guided by the hand-eye synergistic layout method is explored, and the results show that the combination of the spherical interface, the interface spatial morphology layout of the right shoulder reference center, and the interface field of view surface layout of the interaction components in the designated center area and the area below it has a more optimal interaction effect. Thus, it proves the impact of the proposed layout method as a guided interface layout form on the virtual hand interaction performance in 3D space and the usability of the hand-eye collaborative layout to further advance the human-computer interaction performance and experience in virtual environments.