Abstract:
Rapid advances in high-resolution, high-throughput 3D microscopy techniques in the past decade have opened up new avenues for brain research. One such technique developed...Show MoreMetadata
Abstract:
Rapid advances in high-resolution, high-throughput 3D microscopy techniques in the past decade have opened up new avenues for brain research. One such technique developed in our lab is called the Knife-Edge Scanning Microscopy (KESM). The basic principle of KESM is to line-scan image while simultaneously sectioning thin tissue blocks using a diamond microtome. We have successfully sectioned and imaged whole mouse brains and portions of a rat brain processed with different stains to investigate the microstructures within. In this paper, we will present a fully automated soma (cell body) detection method based on random forests, working on Nissl-stained rat brain specimen. The method enables fast and accurate cell counting and density measurement in different brain regions.
Date of Conference: 12-17 July 2015
Date Added to IEEE Xplore: 01 October 2015
ISBN Information: