Identification of carotid plaque motion alterations, throughout the cardiac cycle (CC), may reveal rupture-prone plaque components and assist doctors in ischemic stroke risk stratification. In this study, we investigated Ultrasound (U/S) carotid plaque motion, focusing on middle-systole (MS), the interval between early systole (ES) and peak systole (PS) expecting that the dynamic forces applied on the plaque at the beginning of systole are more profound from cardiac ES to MS, compared to PS. We deployed a computer tool, previously developed by researchers of the e-health laboratory at Cyprus University of Technology, for carotid plaque motion estimation in B-mode U/S videos. We included 16 carotid U/S videos (8 Asymptomatic, AS; 8 Symptomatic, SY patients) and performed video frame (VF) resolution standardization, manual region of interest (ROI) selection, identification of 5 CCs (ES and MS pairs), and plaque motion spread and magnitude measurement, based on dense optical flow. The Maximum Angular Spread (MAXFW20, first 20% of the plaque’s overall MAXFW; units: 0° to 360°), a measure we created to determine angle differences between motion vectors, in all plaque areas, can assist in classifying the plaque as concordant (low-strain), moderate(m)-discordant (mid-strain) or discordant (highstrain). We identified 1 concordant, 8 m-discordant, and 7 discordant plaques, with MAXFW20 ranging from 58±7.45°, 91±43.9°, 156±49.2° (mean±standard deviation), respectively. As our primary findings support our hypothesis, in the future, we will also derive the stress applied on the carotid plaque, in ES and MS.