This paper describes a multiple pitch estimation technique that is based on the bispectrum of the audio signal. Via the bispectrum a 2D (frequency-lag) distribution is computed, that is used for the pitch estimation. The estimated pitch components are then removed from the frequency-lag distribution, recursively, to estimate the subsequent pitch frequencies. The use of two dimensions makes the filtering a relatively easier task than removing components from the one dimensional autocorrelation, as conventionally done. Excellent multiple pitch estimation results are demonstrated via simulations.