This paper presents a CMOS-based lab-on-chip platform for combined magnetic manipulation and opto-chemical sensing. Within each pixel, a Programmable Gate (PG) ISFET chemical sensor is combined with an active pixel sensor, and is encompassed within an inductive coil. The integrated pixel is tesselated to form an 8 × 8 array. Fabricated in a commercially available 0.35 μm CMOS technology, the system can be used for simultaneous optical imaging and pH sensing, and includes auto- calibration mechanisms for eliminating sensor non-idealities. A spatiotemporal magnetic field pattern generator has also been embedded for micro-scale magnetic manipulation. Controlled via a MATLAB based graphical user interface, the system achieves real time data acquisition at 6 fps, a pH sensitivity of 57 mV/pH and demonstrates magnetic manipulation of micro-beads.