Acoustic monitoring of birds in the vicinity of wind turbines is becoming an important public policy issue. Acoustic monitoring involves preprocessing, feature extraction and classification. A novel Spectrogram-based Image Frequency Statistics (SIFS) feature extraction algorithm has been developed. Features extracted from proposed algorithms were then combined with various classification algorithms such as k-NN, Multilayer Perceptron (MLP) and Hidden Markov Models (HMM) and Evolutionary Neural Network (ENN). SIFS and MMS algorithms, combined with ENN, provided the most accurate results. Proposed algorithms were tested with real data collected during spring migration around Lake Erie in Ohio.